Cloning and Expression Analysis of Copper Chaperone Gene CsATX1 from Cucumber
There are many varieties of cucumbers in China,but the resistance of cucumbers is not strong,and they are prone to pest and disease.Therefore analysis and validation of disease resistance related genes in cucumber and exploring its molecular mechanism can provide a theoretical basis for breeding resistant cucumber varieties.In this study,taking cucumber as experimental materials,the CsA TX1 gene cDNA full-length sequence was cloned by RT-PCR technology.Characteristics including the physicochemical properties and conserved domain of the de-duced CsATX1 protein were determined by a series of bioinformatics tools.RT-qPCR technology was performed to measure the transcript levels of CsATX1 gene induced by Pseudomonas syringae pv.lachrymans to clone CsATX1 gene and study the re1ationship between CsATX1 gene and disease resistance of cucumber.The results showed that the full-length nucleotide sequence of CsATX1 was 768 bp,containing a complete open reading frame of 288 bp which encoded a polypeptide of 95 amino acids.Bioinformatics analysis of the amino acid sequence showed that the molecular weight of encoded protein was 9.95 kD,and theoretical isoelectric point was 5.07.This protein was a hydrophilic protein,without transmembrane and signal peptide sequence.Phylogenetic tree analysis indicated that CsATX1 had the closest relationship with the homologous protein of Solanaceae.The expression analysis of the gene by RT-qPCR showed that the CsATX1 expressd in C.sativus leaves.Induced by P.syringae pv.lachrymans,the transcript levels of CsATX1 in cucumber leaves remarkably increased with the extension of induction time.The re-sults of this study provides theoretical basis for further exploring the function of CsATX1 gene.
NETL
NSTL
万方数据
维普
