Genome-wide Identification and Expression Analysis of Durian Acid Inver-tase Family DzAI
In order to investigate the biological functions of the durian DzAI family,we used bioinformatics meth-ods to identify 16 DzAI members from a publicly released high-quality durian genome,of which 8 were cell wall acid convertase and 8 were vacuolar acid convertase.The predicted structure of physical and chemical characteris-tics showed that the isoelectric points of DzAI were different,and other properties were relatively stable.The spa-tial conformation analysis showed that three groups of members had high spatial structure similarity,the random coil is the most in the secondary structure and the secondary structure of DzAI02,DzAI05,DzAI06,DzAIl0,DzA-I13,DzAI14,DzAI15 and DzAI16 contained a transmembrane structure.Phylogenetic tree analysis showed that DzAI could be divided into two subgroups,namely cell wall acid invertase group(subgroup Ⅰ)and vacuolar acid invertase group(subgroup Ⅱ).DzAI01,DzAI03,DzAI04,DzAI05,DzAI07,DzAI08,DzAI10 and DzAI12 were subgroup Ⅰ.DzAI02,DzAI06,DzAI09,DzAI11,DzAI13,DzAI14,DzAI15 and DzAI16 belong to the Ⅱ family.More closely phylogenetically related members are more conserved.Cis-acting element analysis showed that DzAI may be susceptible to light signal,salicylic acid,jasmonic acid and abscisic acid signals.Transcriptome expression analysis showed that DzAI05,DzAI06 and DzAI13 may be related to sugar accumulation in durian fruits.This study provides a scientific basis for further research on DzAI member functions.