Identification and Expression Analysis in Different Tissues of WRKY Tran-scription Factors in Rosa roxburghii Tratt
WRKY transcription factors belong to an important kind of transcription factors in plants,involved in the regulation of plant growth,development and senescence,as well as in plant responses to biotic and abiotic stresses.WRKY gene family members were identified and classified based on transcriptome data from different tissues of Rosa roxburghii Tratt,and their phylogenetic evolution,selective pressure level,conserved domains,and expression in different tissues were also analyzed in this study.A total of 58 WRKY transcription factors were identi-fied,which could be divided into three groups according to the classification method of A rabidopsis thaliana:Ⅰ(11),Ⅱ(36)and Ⅲ(11).The Ⅱ group contained five subgroups:Ⅱ a(3),Ⅱ b(6),Ⅱ c(13),Ⅱ d(7)and Ⅱ e(7),and one gene RrWRKY58 was not included in the three groups.The psequence length of 58 WRKY proteins ranged from 145 to 1 526 amino acids,with molecular weight of 16.94 to 172 842.37 kD and isoelectric point of 4.61 to 9.84.There were mutations in the conserved heptapeptide of WRKYGQK in the WRKY domain of RrWRKY31,RrWRKY32,RrWRKY47,RrWRKY48 and RrWRKY56.The results of selection pressure analysis showed group Ⅰ,Ⅱ a in group Ⅱ and group Ⅲ were under high selective pressure(dn/ds＞1),while Ⅱ b,Ⅱc,Ⅱd and Ⅱe in group Ⅱ were under purfication selection(dn/ds＜1).Expression analysis of the WRKY gene in the stem,leaf,flower and young fruit and mature fruit showed that 45 genes were expressed in all the five tissues to varying degrees(TPM＞0.1).RRW-RKY01,RRWRKY04,RRWRKY06,RRWRKY12,RRWRKY14 and RRWRKY34 were expressed relatively high in all tissues(TPM＞20).These genes may be involved in regulating the growth and development of stem,leaf,flower and fruit.The most abundant genes(TPM＞50)were expressed in stem(12),followed by leaves(8),flowers(7),young fruits(7)and mature fruits(6).This study laid a foundation for further study on the function of WRKY gene in Rosa roxburghii Tratt.
Rosa roxburghii TrattWRKYTranscription factorPhylogenetic evolutionExpression pattern
NETL
NSTL
万方数据
维普
