Cloning,Tissue-specific Expression,and Subcellular Localization Analysis of HbHsfB4b Gene in Rubber Tree
Heat shock transcription factor(Hsf)plays an important regulatory role in plant tissue development,struc-tural development,and abiotic stress response.To investigate the regulatory function of Hsf in resisting adversity.In this study,the full-length open reading frame sequence of HbHsfB4b gene was cloned based on the rubber tree genome database,transient expression vectors were constructed to clarify the subcellular localization,detection its expression in different developing tissues of rubber trees using RT-qPCR.The results showed that the full-length open reading frame of HbHsfB4b gene was 1 080 bp,encoded 359 amino acids,the molecular weight was 40.25 kD and theoretical isoelectric point was 7.28,hydrophilic protein,which consisted of three α-helix and four β-sheet.Sequence alignment analysis showed that the HbHsfB4b protein had high similarity with JcHsfB4(Jatropha curcas)and RcHsfB4(Ricinus communis)proteins,with 91.99％and 87.98％,respectively.The RT-qPCR analysis revealed that the HbHsfB4b gene was most abundantly expressed in the copper-drown stage leaves.This study provides a reference for further research on the biological function of HbHsfB4b gene in rubber trees.
NETL
NSTL
万方数据
维普
