Identification of Hippeastrum spp.Germplasm Resources Based on EST-SSR Marker
Based on the germplasm resources of Hippeastrum spp.introduced over the years,they were grouped according to the varieties with similar flowers and the varieties with the same name.Among them,the varieties with similar flowers included 45 varieties of 12 combinations,and the varieties with the same name included 31 varieties of 12 combinations.Polymorphic primers were screened from the developed EST-SSR markers to iden-tify these introduced Hippeastrum spp.germplasm resources.The results showed that five of the seven pairs of SSR markers developed from the vermilion transcriptome were used for this experiment.Through the cluster analysis of approximate flower combinations,it was found that the genetic similarity coefficient of some vari-eties in five of the 12 combinations was high,and most of the combinations and varieties presented complex ge-netic relationships;clustering results of homonymous combinations showed that 9 of 12 combinations had most varieties clustered together;the genetic background of three combinations of'Orange Sovereign','Apple Blos-som'and'Benfica'was complex.It could be seen from the genetic relationship of some combinations that the ge-netic similarity coefficient of multiple groups of white single-petal and red-white striped single-petal combinations was high,and multiple groups of double-petal and single-petal varieties gather together.The study provides a refer-ence for the study of the genetic relationship between the varieties of Hippeastrum spp.and the identification of the germplasm resources of Hippeastrum spp.