Establishing the System of Detoxification and Rapid Propagation in"Shuicheng Small Yellow Ginger"
To establish a tissue culture and rapid propagation system of"Shuicheng Small Yellow Ginger",taking the shoot tip of"Shuicheng Small Yellow Ginger"bud as the explant,the effect of shoot tip disinfection,callus induction,adventitious bud proliferation,rooting and domestication were studied with different disinfection time,different differentiation medium,different proliferation medium and different rooting medium on the shoot tip disinfection,callus induction,adventitious bud proliferation,and tested the virus-free effect of tissue culture seed-lings.The results showed that the shoot tip of"Shuicheng Small Yellow Ginger"sprouts was sterilized with 75％medical alcohol for 60 s+10％sodium hypochlorite(NaClO)for 20 min,and the effect was the best,with a pol-lution rate of 7.7％and a survival rate of 87.2％.The different medium had the different effects of the shoot tip differentiation,MS+6-BA 3.5 mg/L+NAA 0.1 mg/L had the best effect,and the callus induction rate reached 75.0％.The most suitable proliferation medium was MS+6-BA 3.0 mg/L+NAA 0.3 mg/L which had large proliferation coefficient,vigorous growth,thick stems and strong buds,green leaves,tissue cultured seedlings growed well,and can be coordinated with rooting.The suitable medium for rooting was MS+NAA 0.10 mg/L,with a large number of roots,the average root length was moderate.The double antibody sandwich enzyme-linked immunoassay(ELISA)was used to determine the tobacco mosaic virus(TMV)and cucumber mosaic virus(CMV)in the tissue cultured seedlings,the core plantlets of"Shuicheng Small Yellow Ginger"detoxified was obtained,and the detoxification rate was over 93.75％.Therefore,this study believes that the establishment of a virus-free tissue culture and multiplication system for the shoot tip of"Shuicheng Small Yellow Ginger"can provide references for the detoxification and rapid propagation of"Shuicheng Small Yellow Ginger"seedlings and industrial seedlings.
Small yellow gingerTissue cultureShoot tipDisintoxicationVirus detection
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