Establishment of Somatic Embryogenesis and Plant Regeneration of Cercidiphyllum japonicum
Cercidiphyllum japonicum is a second class protected wild plant of Cercidiphyllum.In this study,a stable and efficient method for somatic embryogenesis and regeneration was established for the first time.Immature embryos were used as explants and cultured on MS medium supplemented with 1.0 mg/L 2,4-D,0.5 mg/L 6-BA for 5 weeks,the callus induction rate was 59.33％,and 100％transformed into embryogenic callus in subsequent proliferation culture.The optimal medium for embryogenic callus proliferation was MS supplemented with 0.1 mg/L NAA,0.1 mg/L 6-BA,0.5 g/L activated carbon and 5％mashed banana,and the growth capacity reached 1.579 7 g.Somatic embryos were differentiated from embryogenic callus in proliferative medium.All embryogenic callus initially produced spherical somatic embryos,which were transformed into heart-,torpedo-and cotyledon-shaped somatic embryos.The seedlings of somatic embryos were eventually differentiated in MS medium without plant growth regulators.After 30 days of culture in MS medium containing 1.0 mg/L IBA,the plants took root and were robust.
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