连香树体细胞胚发生及植株再生体系建立
Establishment of Somatic Embryogenesis and Plant Regeneration of Cercidiphyllum japonicum
樊小宁 1白天宇 1邬洁丽 1杨敬元 2姜志国 2梁宏伟1
作者信息
- 1. 三峡大学,生物技术研究中心,三峡区域植物遗传与种质创新重点实验室,宜昌,443002
- 2. 神农架国家公园管理局,神农架金丝猴保育生物学湖北省重点实验室,神农架林区,442421
- 折叠
摘要
连香树(Cercidiphyllum japonicum)是连香树属的落叶乔木,为中国二级保护植物.本研究首次建立了一种稳定、高效的连香树体细胞胚发生及植株再生的方法.以未成熟种子中的幼胚为外植体,在附加1.0mg/L 2,4-D和0.5mg/L6-BA的MS培养基上培养5周,愈伤组织的诱导率为59.33％,在后续的增殖培养中100％转化为胚性愈伤组织.最适的胚性愈伤组织增殖培养基为附加0.1 mg/L的NAA、0.1 mg/L的6-BA、0.5 g/L活性炭和5％香蕉泥的MS培养基,增殖量达到1.579 7 g.在增殖培养基中胚性愈伤组织实现了体细胞胚的分化发生,胚性愈伤组织最初都产生球形体细胞胚,在培养后期转化为心形期、鱼雷期和子叶期的体细胞胚.子叶期体细胞胚最终在无植物生长调节剂的MS培养基中萌发出苗,在附加1.0 mg/L IBA的MS培养基培养30 d后生根且植株健壮.
Abstract
Cercidiphyllum japonicum is a second class protected wild plant of Cercidiphyllum.In this study,a stable and efficient method for somatic embryogenesis and regeneration was established for the first time.Immature embryos were used as explants and cultured on MS medium supplemented with 1.0 mg/L 2,4-D,0.5 mg/L 6-BA for 5 weeks,the callus induction rate was 59.33％,and 100％transformed into embryogenic callus in subsequent proliferation culture.The optimal medium for embryogenic callus proliferation was MS supplemented with 0.1 mg/L NAA,0.1 mg/L 6-BA,0.5 g/L activated carbon and 5％mashed banana,and the growth capacity reached 1.579 7 g.Somatic embryos were differentiated from embryogenic callus in proliferative medium.All embryogenic callus initially produced spherical somatic embryos,which were transformed into heart-,torpedo-and cotyledon-shaped somatic embryos.The seedlings of somatic embryos were eventually differentiated in MS medium without plant growth regulators.After 30 days of culture in MS medium containing 1.0 mg/L IBA,the plants took root and were robust.
关键词
连香树/体细胞胚胎发生/植物生长调节剂/再生Key words
Cercidiphyllum japonicum/Somatic embryogenesis/Plant growth regulator/Regeneration引用本文复制引用
基金项目
神农架国家公园珍稀濒危植物保育技术研究与示范基金项目(2018ACA132)
出版年
2024
NETL
NSTL
维普
万方数据