Cloning and Identification of Wheat Demethylase Gene DME-5B
Wheat DME protein and rice ROS1 are a DNA demethylase of the same kind.ROS1 has a limiting ef-fect on the number of aleurone layers in rice.Whether DME has a similar function in wheat has not been reported.In this study,the gene TraesDME-5B was cloned from wheat by homologous cloning technique,and the physico-chemical properties and structural characteristics of the protein encoded by TraesDME-5B were analyzed in order to study the effect of demethylase DME on the aleurone layer during wheat seed development.The results showed that the total length of TraesDME-5B was 5 862 bp,encoding 1 953 amino acids,and DME-5B was predicted to be hydrophobic protein.The secondary structure of TraesDME-5B mainly consisted of α-helixes and random coils,among which the latter accounted for 60.73％,playing the role of the most important component in the secondary structure of DME-5B,meanwhile the prediction results of tertiary structure and secondary structure were highly consistent.According to the conserved domains analysis,the DME family contains three conserved domains namely RRM DME,Endo3C superfamily and Perm-CXXC during the evolution of Gramineae.The results showed that TraesDME-5B is a DNA demethylase in wheat,and functions in DNA demethylation.
DNA demethylationWheatHomologous cloningTraesDME-5B
NETL
NSTL
万方数据
