Cloning and Bioinformatics Analysis of the Salt Tolerance Related Gene GbPLA 1-32 in Gossypium barbadense
In order to understand the structural characteristics and functions of the sea island cotton GbPLA 1-32 gene.In this study,the'Hai7124'in the sea island cotton was used as the experimental material,and the differen-tially expressed genes were screened and cloned using the results of salt-tolerant transcriptome sequencing.Through bioinformatics analysis,the full-length CDS of'Hai7124'GbPLA 1-32 gene was obtained.Analyze the physical and chemical properties of the protein encoded by the gene.Identify its hydrophilicity and hydrophobici-ty.Prediction of phosphorylation and glycosylation sites,signal peptides and transmembrane regions,and protein structure.Determine subcellular location and construct a phylogenetic tree.The results showed that the coding re-gion of the gene was 1 530 bp in length,encoding 509 amino acids,and the encoded protein was in the phospholi-pase Al family,belonging to the α/β-hydrolase superfamily.It is a hydrophilic protein,there is no obvious signal peptide and no transmembrane structure,subcellular localization in the chloroplast.At the same time,the four ele-ments of α-helix,extended strand,β-turn and random coil constitute the secondary structure of the GbPLAl-32 protein.The phylogenetic tree analysis showed that the relationship between sea island cotton and upland cotton is the closest.The gene was named GbPLA 1-32 according to its coding protein.The results of RT-qPCR analysis showed that the GbPLA 1-32 gene can respond to salt and alkali stress.The results of this study can provide refer-ences for further elucidating the biological functions of GbPLA 1-32 in Gossypium barbadense.
NETL
NSTL
万方数据
