首页|甜瓜MADS-box基因家族的鉴定及系统进化分析

甜瓜MADS-box基因家族的鉴定及系统进化分析

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中国是甜瓜的主要生产国家,种植面积和产量都位于世界首位.本研究通过全基因组分析鉴定了甜瓜MADS-box基因家族成员,分析甜瓜MADS-box基因家族成员的染色体定位、多重序列比对、motif分布和外显子-内含子结构分布,以及对其成员进行进化及表达分析,并分析启动子区的顺式作用元件分布.结果显示,从甜瓜全基因组数据库中共鉴定了45个甜瓜 MADS-box基因,其中Ⅰ型基因11个,Ⅱ型基因34个;通过系统发育分析,Ⅰ型基因主要分布于Mβ和Mγ亚家族中;Ⅱ型基因则分布于13个亚家族;Ⅰ型基因与Ⅱ型基因的motif分布和外显子个数区别明显,但同型基因区别不大;对CmMADS-box基因家族全生理期表达量进行可视化分析并进行了共线性分析;根据启动子区的顺式作用元件分布情况,推测CmMADS-box基因可能响应多种外界胁迫,即具有功能多样性.该研究结果为探究甜瓜MADS-box基因家族生物学功能提供参考.
Identification and Phylogenetic Analysis of MADS-box Gene Family in Cucumis melo L.
China,in which lies the world's biggest planting area,becoming the main producer of muskmelon and the yield ranks first.Based on genome-wide analysis,we identified the members of MADS-box gene family in melon.The chromosomal location,multiple sequence alignment,motif distribution,exon-intron structure,phyloge-ny,expression pattern and cis-acting elements distribution of MADS-box gene family members were analyzed.The results showed that 45 CmMADS-box genes were identified from the whole genome database of melon,among which there are 11 of type Ⅰ genes and 34 of type Ⅱ genes.According to phylogenetic analysis,type Ⅰ genes are mainly distributed in Mβ and Mγ subfamilies and type Ⅱ genes are distributed in 13 subfamilies.The motif distri-bution and the number of exons of type Ⅰ gene and type Ⅱ gene are different,but the difference of homotype genes is not significant.The expression of CmMADS-box gene family was performed visual analysisduring the whole physiological period.According to the prediction of cis-acting elements in the promoter region,the CmMADS-box gene may respond to a variety of environmental stresses,namely,it has functional diversity.The results of this study provide a reference for further exploring the biological functions of the MADS-box gene family in melons.

MADS-boxCucumis melo L.Gene familyEvolutionary analysisExpression analysis

秦玉杰、任辉、祝建波

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石河子大学生命科学学院,石河子,832000

MADS-box 甜瓜 基因家族 进化分析 表达分析

兵团科技攻关计划

2020AB009

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(5)
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