甜瓜MADS-box基因家族的鉴定及系统进化分析
Identification and Phylogenetic Analysis of MADS-box Gene Family in Cucumis melo L.
秦玉杰 1任辉 1祝建波1
作者信息
- 1. 石河子大学生命科学学院,石河子,832000
- 折叠
摘要
中国是甜瓜的主要生产国家,种植面积和产量都位于世界首位.本研究通过全基因组分析鉴定了甜瓜MADS-box基因家族成员,分析甜瓜MADS-box基因家族成员的染色体定位、多重序列比对、motif分布和外显子-内含子结构分布,以及对其成员进行进化及表达分析,并分析启动子区的顺式作用元件分布.结果显示,从甜瓜全基因组数据库中共鉴定了45个甜瓜 MADS-box基因,其中Ⅰ型基因11个,Ⅱ型基因34个;通过系统发育分析,Ⅰ型基因主要分布于Mβ和Mγ亚家族中;Ⅱ型基因则分布于13个亚家族;Ⅰ型基因与Ⅱ型基因的motif分布和外显子个数区别明显,但同型基因区别不大;对CmMADS-box基因家族全生理期表达量进行可视化分析并进行了共线性分析;根据启动子区的顺式作用元件分布情况,推测CmMADS-box基因可能响应多种外界胁迫,即具有功能多样性.该研究结果为探究甜瓜MADS-box基因家族生物学功能提供参考.
Abstract
China,in which lies the world's biggest planting area,becoming the main producer of muskmelon and the yield ranks first.Based on genome-wide analysis,we identified the members of MADS-box gene family in melon.The chromosomal location,multiple sequence alignment,motif distribution,exon-intron structure,phyloge-ny,expression pattern and cis-acting elements distribution of MADS-box gene family members were analyzed.The results showed that 45 CmMADS-box genes were identified from the whole genome database of melon,among which there are 11 of type Ⅰ genes and 34 of type Ⅱ genes.According to phylogenetic analysis,type Ⅰ genes are mainly distributed in Mβ and Mγ subfamilies and type Ⅱ genes are distributed in 13 subfamilies.The motif distri-bution and the number of exons of type Ⅰ gene and type Ⅱ gene are different,but the difference of homotype genes is not significant.The expression of CmMADS-box gene family was performed visual analysisduring the whole physiological period.According to the prediction of cis-acting elements in the promoter region,the CmMADS-box gene may respond to a variety of environmental stresses,namely,it has functional diversity.The results of this study provide a reference for further exploring the biological functions of the MADS-box gene family in melons.
关键词
MADS-box/甜瓜/基因家族/进化分析/表达分析Key words
MADS-box/Cucumis melo L./Gene family/Evolutionary analysis/Expression analysis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据