Cloning and Expression Pattern Analysis of Tk-bZIP11 Transcription Factor from Taraxacum kok-saghyz Rodin
Taraxacum kok-saghyz is often affected by drought and other stresses during the planting process,and improving the stresses resistance of the variety is of great significance.To investigate the functions of bZIP tran-scription factors in response to stress conditions,the Tk-bZIP11 gene was cloned from Taraxacum kok-saghyz.The ORF of Tk-bZIP11 is 468 bp in length and encodes 156 amino acids.Protein structural analysis showed that Tk-bZIP11 protein contained a conserved domain specific to the bZIP transcription factors,and a nuclear localization signal.In terms of evolutionary relationship,Tk-bZIP1 1 showed the highest similarity to Lactuca sativa Ls-bZIP11,and they contained the same conserved motifs.Subcellular localization indicated that Tk-bZIP11 was localized to the nucleus.The results of RT-qPCR analysis showed that Tk-bZIP1 1 was expressed in different tissues of Taraxacum kok-saghyz,with the highest expression in the leaves,which was 3.28 times higher than those in the stalks.Under osmotic stress induced by mannitol,Tk-bZIP11 expression showed a significant down-regulated after treatment.Under stress conditions treated by NaCl and PEG-4000,Tk-bZIP11 expression was obviously up-regulated within the 12 h of treatments,but down-regulated after 24 h.Analogously,after application of phytohormones(i.e.,methyl jasmonate,ethephon and abscisic acid),Tk-bZIP11 expression was also significantly up-regulated within the 12 h of treatments,and then down-regulated.The above findings suggestted that Tk-bZIP11 was involved in the re-sponse to stress and hormonal signaling processes in Taraxacum kok-saghyz.This study provided a scientific basis and theoretical foundation for studying the mechanism of bZIP transcription factors in response to stress,and creat-ing new germplasm resistant to stress in Taraxacum kok-saghyz.
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