Genetic Diversity Analysis and DNA Fingerprint Construction of Chinese Bayberry Germplasm Resources
In order to analyze the genetic diversity of bayberry germplasm resources,reveal their genetic relation-ship,and promote the effective identification,utilization and protection of them,in this study,83 Chinese bayberry germplasm resources were utilized for genetic diversity analysis by using 20 pairs of InDel primers.Besides,DNA fingerprint map of Chinese bayberry germplasm resources was constructed.The results showed that a total of 110 alleles were detected using 20 pairs of InDel primers,with an average of alleles of per primer were 5.50,and effective alleles of per primer were 4.31,respectively.Nei's genetic diversity index was 0.34,and Shannon's index was 1.42,respectively.The average polymorphic information content was 0.29,and the labeling index was 1.60.83 Chinese bayberry germplasm resources were divided into three groups(including six subgroups),among which group A con-tamed the germplasm that originated from northern coastal of Zhejiang Province and Jiangsu Province,Group B contained the germplasm that originated from the central Zhejiang Province,Group C contained the germplasm that originated from the southwest of Zhejiang Province and Fujian Province.The domestication of Zhejiang Chi-nese bayberry germplasm corresponded to the topographic changes in Zhejiang Province,which indicated that the domestication of Zhejiang germplasm was significantly affected by natural selection of native topography.Based on the polymorphism information content and marker index of primers,the usage of primers was further simpli-fied.9 core InDel primers were screened,and DNA fingerprints of 83 Chinese bayberry germplasm resources were constructed.The research results will help lay the foundation for the standardized construction of a DNA molecular identity card library for bayberry,and provid a certain basis for the scientific utilization and effective protection of bayberry germplasm resources.
Chinese bayberryGermplasm resourcesInDel primersGenetic diversityDNA fingerprint
NETL
NSTL
万方数据
