Regulatory Effect of Biochanin A on Energy Metabolism in Rats with Exercise-induced Fatigue
The purpose of this study was to explore the anti-exercise-induced fatigue(EIF)and energy metabolism regulation of Biochanin A(Bio-A).In this study,the EIF rat model was induced by weight-bearing exhaustive swimming.During exercise,rats were given intragastric administration of three doses of Biochanin A(10,20 and 40 mg·kg-1·d-1),and intraperitoneal injection of 0.5 mg·kg-1·d-1 Compound C(AMPK inhibitor).Exercise or administration for 4 weeks.The exhaustion time,serum harmful metabolites,energy metabolism and oxidative stress of skeletal muscle were measured.The gene transcription level of energy metabolism in skeletal muscle was detected by RT-qPCR.The phosphorylation level of AMPKα in skeletal muscle was detected by Western blot.The results showed that Biochanin A increased the exhaustion time of EIF rats in a dose-dependent manner,decreased the levels of LDH,CK and cTnl,increased the levels of liver glycogen,muscle glycogen and Glu,decreased the levels of LA and BUN,increased the levels of SOD and CAT,decreased the level of MDA,increased the mRNA relative expression of PGC-1α,HK2,GLUT-4 and CS,decreased the mRNA relative expression of PDK4 and increased the relative phosphorylation level of AMPKα(P＜0.05).Compound C attenuated the anti-fatigue effect of Biochanin A(P＜0.05).In a word,Biochanin A can improve the energy metabolism of exercise-induced fatigue rats by activating AMPK pathway and play an anti-fatigue effect.This finding may facilitate further investigation of chickresin A as a potential natural drug to prevent and alleviate exercise fatigue.
Biochanin AExercise fatigueEnergy metabolismAMP activated protein kinaseAntioxidation
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