植物源芥子酸联合运动对非酒精性脂肪肝大鼠的影响
Impact of Plant-Derived Sinapinic Acid Combined with Exercise on Non-Alcoholic Fatty Liver in Rats
皇甫立慧 1李海伟 1魏作富 2戚孙恺3
作者信息
- 1. 山西师范大学体育学院,太原,030031
- 2. 山西师范大学生命科学学院,太原,030031
- 3. 安徽农业大学体育部,合肥,230036
- 折叠
摘要
本研究探讨了植物来源的芥子酸与运动联合治疗对非酒精性脂肪肝(NAFLD)大鼠的脂质代谢基因转录的影响.本研究使用高脂饮食(HFD)诱导NAFLD大鼠模型,将其分为不同干预组,包括单独应用芥子酸、单独进行运动、芥子酸联合运动干预,以及对照组.研究结果显示,芥子酸联合运动治疗显著减轻了NAFLD大鼠的体重,改善了肝功能,降低了肝脂肪含量.在分子水平上,芥子酸联合运动组中,脂质代谢基因SREBP1c、FAS、ACC-1的mRNA水平显著下调,CPT-1、CYP7A1、FXR的mRNA水平显著上调,从而纠正了肝脏脂质代谢基因转录紊乱.此外,芥子酸联合运动组的抗氧化酶SOD和CAT活性明显增加,氧化损伤指标MDA水平降低,炎症因子TNF-α、IL-1β和IL-6水平明显降低.这一综合效应表明,植物来源的芥子酸联合运动治疗通过多重机制改善NAFLD的病理过程.这一研究结果为开发NAFLD治疗策略提供了有力的支持.
Abstract
This study investigated the impact of combined treatment with plant-derived sinapinic acid and exer-cise on the transcription of lipid metabolism genes in non-alcoholic fatty liver disease(NAFLD)rats.An NAFLD rat model was induced using a high-fat diet(HFD)and divided into different intervention groups,including single applications of sinapinic acid,exercise,combined sinapinic acid and exercise,and a control group.The results demonstrated that the combined treatment significantly reduced body weight,improved liver function,and de-creased hepatic fat content in NAFLD rats.At the molecular level,in the sinapinic acid combined with exercise group,mRNA levels of lipid metabolism genes,including SREBP1c,FAS,and ACC-1,were significantly down-regulated,while mRNA levels of CPT-1,CYP7A1,and FXR were significantly upregulated,thereby correcting the transcriptional dysregulation of hepatic lipid metabolism genes.Furthermore,the combined treatment group exhib-ited significantly increased activities of the antioxidant enzymes SOD and CAT,decreased levels of the oxidative damage marker MDA,and a significant reduction in inflammatory factors TNF-α,IL-1β,and IL-6.This compre-hensive effect indicates that plant-derived sinapinic acid combined with exercise treatment improves the pathologi-cal processes of NAFLD through multiple mechanisms.These findings provide strong support for the development of NAFLD treatment strategies.
关键词
小亚麻子/芥子酸/运动/非酒精性脂肪性肝/脂质代谢基因/基因转录Key words
Brassica juncea/Sinapinic acid/Exercise/Non-alcoholic fatty liver disease/Lipid metabolism genes/Gene transcription引用本文复制引用
基金项目
山西省教学改革项目(J20220468)
教育部项目(21YJC890048)
出版年
2024
NETL
NSTL
万方数据