首页|植物源性丹酚酸B对递增负荷训练大鼠骨骼肌铁死亡通路的影响

植物源性丹酚酸B对递增负荷训练大鼠骨骼肌铁死亡通路的影响

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研究旨在探究植物源性丹酚酸B对递增负荷训练大鼠骨骼肌铁死亡通路的影响及其潜在的调控机制.本研究从草本植物丹参中提取分离得到纯化的丹酚酸B,按照实验设计喂食给大鼠,随后对大鼠进行递增负荷训练模拟了运动性疲劳状态,通过分析血清标志物和组织生化指标,研究了丹酚酸B在运动性疲劳过程中对氧化应激和铁代谢的影响.研究发现,丹酚酸B可剂量依赖性地减轻递增负荷训练引起的大鼠骨骼肌损伤,其中血清LDH和CK水平分别降低了50％和45％.丹酚酸B显著降低了骨骼肌中MDA水平,减轻了氧化应激.在铁代谢方面,丹酚酸B升高了骨骼肌中GSH水平,并上调了GPX4的转录和蛋白表达水平,抑制了铁死亡.丹酚酸B对铁代谢相关基因的转录和蛋白表达产生明显的影响,降低了Tf、TfR、DMT1的表达水平,提高了FTH1和FPN1的表达水平.结果显示,丹酚酸B通过调控铁代谢通路,参与抗运动性疲劳过程,为丹酚酸B作为天然抗氧化剂和铁死亡抑制剂的潜在机制提供了新见解.
Impact of Plant-Derived Salvianolic Acid B on Ferroptosis Pathway in Rats Skeletal Muscle Induced by Incremental Load Training
This study aimed to investigate the effects of plant-derived salvianic acid B on the iron death pathway in the skeletal muscles of rats subjected to incremental load training and explore its potential regulatory mecha-nisms.In this study,salvianic acid B was purified from the herbal plant Danshen,and it was administered to rats following an experimental design.The rats were then subjected to incremental load training to simulate the state of exercise-induced fatigue.The study analyzed serum markers and tissue biochemical indicators to examine the im-pact of salvianic acid B on oxidative stress and iron metabolism during exercise-induced fatigue.The results re-vealed that salvianic acid B dose-dependently alleviated skeletal muscle damage induced by incremental load training,reducing serum LDH and CK levels by 50％and 45％,respectively.Salvianic acid B significantly de-creased MDA levels in skeletal muscles,mitigating oxidative stress.In terms of iron metabolism,salvianic acid B elevated GSH levels in skeletal muscles and upregulated the transcription and protein expression of GPX4,inhibit-ing iron death.Salvianic acid B had a noticeable impact on the transcription and protein expression of iron metabolism-related genes,lowering the expression levels of Tf,TfR,and DMT1,while increasing the expression levels of FTH1 and FPN1.The results indicate that salvianic acid B participates in the process of combating exer-cise-induced fatigue by modulating the iron metabolism pathway,providing new insights into the potential mecha-nisms of salvianic acid B as a natural antioxidant and iron death inhibitor.

Salvia miltiorrhiza BungeSalvianolic acid BExercise fatigueIncremental load trainingFerroptosis pathway

王晓娜

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郑州工商学院体育学院,郑州,451400

丹参(Salvia miltiorrhiza Bunge) 丹酚酸B 运动性疲劳 递增负荷训练 铁死亡通路

河南省科技厅科技攻关计划河南省高等学校重点科研项目河南省教育厅人文社会科学研究项目

21210231026721A8900052022-ZZJD-406

2024

10.13271/j.mpb.022.001645

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子：0.765
ISSN：1672-416X
年,卷(期)：2024.22(5)
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