木材的形成基于维管形成层细胞的分裂分化,其中次生壁加厚是关键步骤.研究表明,植物在盐胁迫下细胞壁的组分与结构将发生变化,其中细胞壁表面的多糖阿拉伯半乳聚糖(arabinogalactan protein,AGP)起重要作用.AGP糖链是由糖基转移酶(glycosyltransferase,GT)催化合成,在细胞壁的生物合成中起关键作用,而且其表达量变化直接影响木质部的发育.本研究通过对杨树GT14(Glycosyltransferase family 14)家族进化关系与基因结构分析发现该家族基因在进化上具有较强的保守性.同时,3个杨树GT14基因在盐胁迫下表达量上调,且其中2个基因在体外表现出GlcAT活性.启动子元件分析发现这3个基因均包含多个非生物胁迫响应元件.本研究分析了杨树GT14基因功能,并为提高木本植物非生物耐逆作用提供线索.
Analysis of Poplar Glycosyltransferase GT 14 Gene Family and Screening in Response to Salt Stress
Wood formation is based on the division and differentiation of vascular cambium cells,where the sec-ondary cell wall formation is the key step.It is demonstrated that the structure and composition of the cell wall are al-tered under salt stress,with the cell surface arabinogalactan protein(AGP)playing an important role.Glycosyltransfe-rases(GTs)play a key role in the biosynthesis of the cell wall polysaccharides as well as AGP glycan,therefore signifi-cantly affect xylem development.In this study,we analyzed the phylogenetic relationship and gene structure of GT 14 family in poplar,and these genes were highly conserved in evolution.Three GT14 genes were highly up-regulated un-der salt stress and two genes'enzymes demonstrated GlcAT activity in vitro.Promoter element analysis showed that these three genes contained multiple abiotic stress responsive elements.This study provides the insights of polar GT 14 functionality and brings perspectives of strategies in abiotic stress tolerance improvement in woody crops.
PoplarGlycosyltransferase family 14Phylogenetic analyzeSalt stress
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