Cloning and Bioinformatics Analysis of Auxin Input Gene GzLAX3-1B of'Guizimai No.1'
In order to study the function of auxin gene LAX3 in wheat growth and development.In this study,the full-length cDNA sequence of GzLAX3-1B gene was obtained from'Guizimai No.1'by RT-PCR,and the bioinfor-matics analysis of the gene and its encoded protein was carried out.The results showed that the open reading frame(ORF)of GzLAX3-1B gene was 1 587 bp,encoding 528 amino acids,with PLN03074 specific site,belonging to SLC5-6-like-sbd superfamily.It encodes a protein with 44 phosphorylation sites and 10 transmembrane structures,which is an unstable hydrophobic plasma membrane protein without signal peptide.The results of phylogenetic anal-ysis showed that the GzLAX3-1B gene of'Guizimai No.1'was closely related to wild emmer wheat,barley and oil palm.The results of this study provide a theoretical basis for further study on biological function of wheat LA X.
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