摘要
为探究红光促进棉花胚性愈伤组织形成的分子机理.本研究以陆地棉CCRI24为试验材料,利用RNA-seq技术对在红光、白光培养条件下的不同阶段棉花愈伤组织进行转录组测序,分析胚性愈伤形成过程的基因表达水平.对2个不同光处理愈伤组织共计8个样品进行转录组测序,共获得54.92 Gb clean data.与白光相比,5、15、20、30d的非胚性愈伤组织中差异表达基因分别有261、58、139、44个上调,454、157、328、507个下调.GO功能富集分析表明,两种光质条件下的棉花愈伤组织差异表达基因的功能主要富集到细胞核、细胞膜等与细胞分裂有关的代谢过程.KEGG富集分析结果显示,红光与白光条件下的愈伤组织间差异表达基因主要参与光合作用-天线蛋白、玉米素合成、二萜类生物合成、类黄酮生物合成等代谢通路.研究结果表明,与胚性愈伤组织形成相关的8个基因的差异表达趋势与转录组测序分析结果一致.本研究为光质影响棉花胚性愈伤诱导的相关基因表达提供了数据,为解析棉花胚性愈伤组织诱导与形成的分子机制提供参考.
Abstract
The purpose of this study was to explore the molecular mechanism of red light promoting the forma-tion of cotton embryogenic callus.In this study,upland cotton CCRI24 was used as the experimental material,and transcriptome sequencing was performed in different stages of cotton callus cultured under red light and white light by RNA-seq technology to analyze the gene expression level in embryogenic callus formation process.Transcrip-tome sequencing was performed on 8 samples from 2 different light-treated callus,and 54.92 Gb of clean data was obtained.Compared with white light,261,58,139 and 44 differentially expressed genes were up-regulated and 454,157,328 and 507 differentially expressed genes were down-regulated in non-embryonic callus at 5,15,20 and 30 d,respectively.The GO function enrichment analysis showed that the functions of differentially expressed genes in cotton callus under two light quality conditions were mainly concentrated in the metabolic processes rela-ted to cell division,such as nucleus and cell membrane.KEGG enrichment analysis showed that the differentially expressed genes in red and white light callus were mainly involved in photosynthesis-antenna protein,zeatin biosynthesis,diterpenoid biosynthesis,flavonoid biosynthesis and other metabolic pathways.In this study,the dif-ferential expression trend of 8 genes related to embryogenic callus formation was consistent with the results of transcriptome sequencing analysis.This study provided data on the effect of light quality on gene expression of embryogenic callus induction in cotton,and provided an important basis for the analysis of the molecular mecha-nism of embryogenic callus induction and formation in cotton.
基金项目
国家重点研发计划项目(2016YFD0100505)
国家自然科学基金(31701474)
NETL
NSTL
万方数据