Transcriptome Analysis of Embryogenic Callus Induced by Red Light in Cotton
The purpose of this study was to explore the molecular mechanism of red light promoting the forma-tion of cotton embryogenic callus.In this study,upland cotton CCRI24 was used as the experimental material,and transcriptome sequencing was performed in different stages of cotton callus cultured under red light and white light by RNA-seq technology to analyze the gene expression level in embryogenic callus formation process.Transcrip-tome sequencing was performed on 8 samples from 2 different light-treated callus,and 54.92 Gb of clean data was obtained.Compared with white light,261,58,139 and 44 differentially expressed genes were up-regulated and 454,157,328 and 507 differentially expressed genes were down-regulated in non-embryonic callus at 5,15,20 and 30 d,respectively.The GO function enrichment analysis showed that the functions of differentially expressed genes in cotton callus under two light quality conditions were mainly concentrated in the metabolic processes rela-ted to cell division,such as nucleus and cell membrane.KEGG enrichment analysis showed that the differentially expressed genes in red and white light callus were mainly involved in photosynthesis-antenna protein,zeatin biosynthesis,diterpenoid biosynthesis,flavonoid biosynthesis and other metabolic pathways.In this study,the dif-ferential expression trend of 8 genes related to embryogenic callus formation was consistent with the results of transcriptome sequencing analysis.This study provided data on the effect of light quality on gene expression of embryogenic callus induction in cotton,and provided an important basis for the analysis of the molecular mecha-nism of embryogenic callus induction and formation in cotton.
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