The Stress Response Mechanism of Rhus chinensis to Schlechtendalia chin-ensis Based on Comparative Transcriptome
Galla chinensis is one of the commonly used Chinese medicinal materials,which is formed by Schlech-tendalia chinensis infection on its host plant Rhus chinensis.At present,there are few studies on the stress re-sponse mechanism of R.chinensis to S.chinensis during the formation of Galla chinensis.In this study,the gall and non-gall leaves of Rhus chinensis were used as materials to study the stress response mechanism of Rhus chi-nensis toS.chinensis during the formation of Galla chinensis by using a new generation of transcriptome sequenc-ing technology.A total of 26 117 differentially expressed genes(DEGs)were obtained,among which 21 581 genes were up-regulated and 4 536 genes were down-regulated.Through the GO and KEGG database,the DEGs were mainly concentrated in the pathways of substance synthesis and metabolism,genetic material changes,plant hor-mones and photosynthesis.In this study,we screened a total of 292 DEGs in plant hormone synthesis and signal transduction pathway,ethylene(ET)synthesis and signal transduction pathway showed the most significant dif-ferences,which were closely related to stress response.CTR1 and MPK6 are key genes in ET signal transduction pathway which were significantly different expressed between the two materials.In addition,a total of 381 differ-entially expressed transcription factors were obtained through transcription factor annotation,among which,we screened 83 significantly expressed transcription factors closely related to plant response,including MYB,bHLH,AP2-EREBP,NAC,WRKY and bZIP.In this study,the second-generation high-throughput transcriptome sequenc-ing technology was used to study the expression characteristics of genes related to the stress response of S.chinensis infection,which laid a foundation for the future study of the stress mechanism of the response of the Galla chinensis to the S.chinensis and the molecular mechanism of the Galla chinensis formation.
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