Screening and Analysis of Photosynthetic Pigment Related Genes in Acer pseudosieboldianum Based on Transcriptome Sequencing
In order to reveal the intrinsic molecular mechanism of photosynthetic pigment in Acer pseu-dosieboldianum,the functional genes closely related to photosynthetic pigments were explored.Based on the tran-scriptional data of Acer pseudosieboldianum,we locate the detected Unigene to specific metabolism pathway branches.We summarize KEGG annotation number(KO)related to photosynthetic pigment.We can get up to 27 pathway branches of porphyrin and chlorophyll metabolism pathway(ko00860),and 17 pathway branches of carotenoid biosynthesis pathway(ko00906).66 differential expression gene(DEGs)related to photosynthetic pig-ment were screened out,28 of them were up regulated and 38 were down regulated.The selected photosynthetic pigment related pathways,DEGs,were predicted.28 structural genes were screened out in 66 DEGs,and 19 key enzymes(POR,DVR,NOL,LCYB,NCED,CYP707A)were selected from 66 DEGs,and they can be divided into four types:chlorophyll biosynthesis,chlorophyll degradation,carotenoid synthesis and carotenoid degradation.The screened structural genes were compared with the protein sequences in the KOG database and the GO func-tion classification statistics were carried out.The most abundant ones were biological process,among which the most significant functional enrichment was related to metabolism process,single biological process and cell pro-cess.The screening of photosynthetic pigment related genes will help to reveal the molecular mechanism of leaf color formation of Acer pseudosieboldianum,and provide data support for the future study on gene localization and gene cloning of Acer pseudosieboldianum.
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