Identification and Expression Analysis of MADS-box Transcription Factor Family in Cyperus esculentus
The MADS-box gene family is intimately related to growth and regulation of development in plant.In the light of the results of RNA-Seq for tuber of Cyperus esculentus,the results of this research were as follows.To proclaim the expression pattern of MADS-box family members.26 family members were identified by HMM search,phylogenetic and conserved domain analysis showed that MADS-box members of Cyperus esculentus be-longed to MIKC type,which has a typical MEF2 like domain.There were 7 expression patterns in different tuber development stages,CeMADSXAL1 gene was highly expressed at the early stage of tuber development.It can be inferred that CeMADSXAL1 gene plays an extraordinary important role in the development of Cyperus esculentus tuber.In the meantime,the CeMADSXAL1 gene was cloned and an open reading frame of 747 bp was obtained,which encoded 249 amino acids,CeMADSXAL1 was found strongly signal in nucleus,that result of the subcellular localization analysis.This study was concluded the theoretical basis for further elucidating the function of MADS-box gene in Cyperus esculentus.
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