陆地棉光合系统Ⅱ GhPsbS基因功能初步探究
Preliminary Study on the Function of Ⅱ GhPsbS Gene in Photosynthetic Sys-tem of Upland Cotton
袁玥 1赵龙飞 1赵瑞海 1李志博 1魏亦农1
作者信息
- 1. 石河子大学农学院,石河子,832000
- 折叠
摘要
为探究棉花光合系统Ⅱ基因GhPsbS,在不同温度条件下的基因相对表达量以及不同温度对野生型和转基因烟草植株的影响.本研究利用转基因GhPsbS烟草(Nicotiana tabacum xanthin)T4代为材料,在生长50d左右,取生长状况相同的转基因植株60株,在光照培养箱中,温度设为35 ℃处理0、1、3、6、18、36h,分别取样,提取RNA,反转cDNA,采用qPCR技术分析转基因植株在不同温度处理下的基因相对表达量并采用LI-6400型便携式光合系统仪(美国Li-cor公司)测量不同处理时间的烟草的光合特性.结果表明:在35 ℃温度处理条件下,抑制该基因的相对表达量.通过对光合数据分析可知,转基因烟草植株的气孔导度(Gs)、蒸腾速率(Tr)呈现明显升高趋势.在高温条件下转基因植株的净光合速率高于野生型.而温度对于转基因和野生型胞间CO2浓度(Ci)关系不大.推测棉花GhPsbS基因参与调控烟草植株的光合作用.研究结果为探究如何提高棉花光能利用效率及增加作物产量提供一定的理论基础.
Abstract
In order to explore the cotton photosynthetic system Ⅱ gene GhPsbS,the relative gene expression under different temperature conditions and the effect of different temperatures on wild-type and transgenic tobacco plants.This study uses the T4 generation of transgenic GhPsbS tobacco(Nicotiana tabacum xanthin)and wild-type tobacco plants(common wild-type,WT)as materials.In a light incubator,when the transgenic plants are significantly different from the wild-type plants(grow to 50 days around),take 60 transgenic and wild-type plants with the same growth status,set the temperature to 35 ℃,and treat them for 0,1,3,6,18,36 h,3 replicates for each treatment,take samples separately,and place the samples put it in the refrigerator at-80 ℃ for later use.Using qPCR tech-nology to analyze the relative gene expression levels of the two under different temperature treatments and the lev-el of expression levels in different parts of the cotton plant.The LI-6400 portable photosynthetic system instru-ment(Li-cor,USA)was used to measure the photosynthetic characteristics of tobacco with different treatment times.The results showed that the relative expression of the gene was inhibited under the condition of 35 ℃ tem-perature treatment.Through the analysis of photosynthetic data,it can be seen that the stomatal conductance(Gs)and transpiration rate(Tr)of transgenic tobacco plants show a significant increasing trend.Under high temperature con-ditions,the net photosynthetic rate of transgenic plants is higher than that of wild type.The temperature has little relationship with the intercellular CO2 concentration(Ci)of transgenic and wild-type cells.It is speculated that the cotton GhPsbS gene is involved in regulating the photosynthesis of tobacco plants.The research results provide a theoretical basis for exploring how to improve the efficiency of cotton light use and increase crop yield.
关键词
棉花/烟草/温度/光合作用/GhPsbS基因Key words
Cotton/Tobacco/Temperature/Photosynthesis/GhPsbS gene引用本文复制引用
出版年
2024
NETL
NSTL
万方数据