The Breeding of New Japonica Rice Varieties with Good Quality by Pyra-miding Wx-mp,fgr,Pi-ta and Pi-b Genes
In this study,the high yield rice line'Ning7020'contained blast resistance gene Pi-b,low-amylose con-tent gene Wx-mp and fragrance gene fgr were used as receipt to be crossed with the rice variety'Yangjing 9538',which carries the blast resistance gene Pi-ta.Self-designed multiple PCR system to detect Pi-ta and Pi-b and te-tra-primer amplification refractory mutation PCR system to detect Wx-mp and InDel marker for fgr were used to detect the target loci in separation generation.Combining with resistance identification,fragrance characterization and classification of the grain endosperm appearance,a new japonica rice line'Nanjing58'with excellent agro-nomic traits was bred by multi-generational selection on resistance,fragrance and endosperm appearance.This study provides a simple and fast selection technique for breeding new varieties of multi-resistant and high-quality rice,and also provides new germplasm resources for rice disease resistance and high-quality breeding.
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