An Improved Method of the Whole Staining and Clearing Technique to Ob-serve the Nucellus of Cotton
Cotton has a larger floral organ.However,due to the thick bead structure,modem microscopes,such as laser confocal microscopy,are difficult to observe the specific process of female gametophyte directly,which af-fects its research progress.To observe the process of the cotton reproductive development process,this study ob-served the fertilization process of cotton by stripping the nucellus for whole staining and transparency.Cotton nu-cellus were stained with Azure C of different concentrations,and the nucellar tissue was clearly treated with Birch-Me(BM)to observe female gametophyte.The results showed that this method can clearly distinguish the microstructures of the cell membrane,nucleus,and nuclear membrane.This method only needed simple experi-mental equipment and a small amount of manual operation to observe the cell of cotton and other plants such as cotton for large embryo sac tissue.,At the same time,it provides a simple and effective method for the study of cotton reproductive development.
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