Joint Multi-generation Genetic Analysis on Chilling Tolerance of Bitter Gourd Seedlings Stage
In order to deeply understand the genetic law and effect of resistance to chilling injury in bitter gourd,we constructed the genetic population of six generations of P1,P2,F1,BCP1,BCP2 and F2 used inbred line'Y45'and'Y51'as the male and female parent respectively.In the seedlings,the study carried out genetic analysis of cold tol-erance by using the mixed genetic model of quantitative trait major genes and polygenes.The results showed that the cold tolerance of Momordica charantia L.in seedling stage was in accordance with two pairs of additive domi-nant epistasis major genes × additive dominant polygenes mixed genetic model.The additive effect value of the two main genes of cold resistance was-13.57,and the dominant effect values were-2.63 and-2.71,this result il-lustrated that it is practicable to carry out genetic improvement of cold tolerance by seedlings.The heritability of major genes in BCP1,BCP2 and F2 segregating generations were 75.11%,73.96%and 91.13%.The variation caused by environmental factors accounted for respectively in the three segregating generations 17.63%,15.11%和8.87%.This study showed that cold tolerance breeding of bitter gourd could be mainly selected from F2 and selfing progenies and provided a reference for the breeding of bitter gourd seedlings.