苦瓜苗期耐冷性的多世代联合遗传分析
Joint Multi-generation Genetic Analysis on Chilling Tolerance of Bitter Gourd Seedlings Stage
牛玉 1于仁波 1韩旭 1刘昭华 2杨衍1
作者信息
- 1. 中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创新重点开放实验室,儋州,571737;海南崖州湾种子实验室,三亚,572025
- 2. 中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创新重点开放实验室,儋州,571737
- 折叠
摘要
为深入了解苦瓜对冷害的抗性遗传规律和抗性遗传效应,本研究以自交系'Y45'为父本,冷敏型苦瓜自交系'Y51'为母本,构建P1、P2、F1、BCP1、BCP2和F2这6世代群体遗传群体,采用数量性状主基因+多基因混合遗传模型分析方法对苦瓜苗期耐冷抗性进行遗传分析.研究结果表明苦瓜苗期耐冷符合2对加性-显性-上位性主基因+加性-显性多基因混合遗传模型.耐冷抗性的2对主基因加性效应值均为-13.57,显性效应值分别为-2.63和-2.71,说明开展苦瓜幼苗耐冷遗传改良是可行的.BCP1、BCP2和F2分离世代群体主基因遗传率分别为75.11％、73.96％和91.13％,环境因素引起的变异在3个分离世代群体中分别占17.63％、15.11％和8.87％,该结果表明苦瓜幼苗耐冷性选育可多在F2及自交后代中选择.本研究为苦瓜幼苗耐冷育种提供了参考.
Abstract
In order to deeply understand the genetic law and effect of resistance to chilling injury in bitter gourd,we constructed the genetic population of six generations of P1,P2,F1,BCP1,BCP2 and F2 used inbred line'Y45'and'Y51'as the male and female parent respectively.In the seedlings,the study carried out genetic analysis of cold tol-erance by using the mixed genetic model of quantitative trait major genes and polygenes.The results showed that the cold tolerance of Momordica charantia L.in seedling stage was in accordance with two pairs of additive domi-nant epistasis major genes × additive dominant polygenes mixed genetic model.The additive effect value of the two main genes of cold resistance was-13.57,and the dominant effect values were-2.63 and-2.71,this result il-lustrated that it is practicable to carry out genetic improvement of cold tolerance by seedlings.The heritability of major genes in BCP1,BCP2 and F2 segregating generations were 75.11％,73.96％and 91.13％.The variation caused by environmental factors accounted for respectively in the three segregating generations 17.63％,15.11％和8.87％.This study showed that cold tolerance breeding of bitter gourd could be mainly selected from F2 and selfing progenies and provided a reference for the breeding of bitter gourd seedlings.
关键词
苦瓜/苗期/耐冷/遗传分析Key words
Bitter gourd(Momordica charantia L.)/Seedlings stage/Chilling tolerance/Genetic analysis引用本文复制引用
基金项目
海南省重点研发计划项目(ZDYF2020059)
海南省重大科技计划项目(ZDKJ2021010)
海南崖州湾种子实验室揭榜挂帅项目(B21HJ0305)
海南省重点研发计划项目(ZDYF2020063)
出版年
2024
NETL
NSTL
万方数据