Effects of Different Culture Conditions and Storage Methods on the Pollen Germination in Vitro of Camellia rostrata
The study aimed to find out the viability,suitable medium and storage characteristics of pollen of Camellia rostrata.In this study,the pollen of Camellia rostrata was used as the experimental material.Different su-crose concentration(5％,10％,15％and 20％),boracic acid concentration(100,200,300 and 400 mg/L),culture temperature(4 ℃,18 ℃,26 ℃ and 35 ℃),moisture conditions(28.31％,50.00％and 71.48％),storage temperature(room temperature,4 ℃,-18 ℃ and-80 ℃),and storage time(1,5,10,15,30,60,90,120 days)were set,to find out the effects of different culture conditions and storage methods on the pollen germination in vitro of Camellia rostrata.The results showed that the highest rate of pollens germination was 10％sucrose+100 mg/L H3BO3.The pollens fully germinated when they were cultured at 26 ℃ for 1.5 hours.The active ability of pollen would be re-duced when the pollen treated by low temperature with water content rate of 50.00％and 71.48％.The effect of-80℃ cryopreservation was better than 4 ℃ cold storage with water content rate of 28.31％,the germination rate of pollens was 36.75％after stored 120 days.The best medium for pollen germination was 10％sucrose+100 mg/L H3BO3,proper drying and low temperature were favorable for the long-term preservation of the pollen of Camel-lia rostrata.The results could provide a theoretical basis for preserving the pollen of Camellia rostrata and improv-ing the success of crossbreeding of Camellia rostrata.
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