首页|板栗花药发育过程中的组织化学观察

板栗花药发育过程中的组织化学观察

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以不同发育阶段的板栗雄花为试验材料,通过组织化学方法对板栗花药发育中淀粉、多糖、蛋白质积累和分布进行观察.结果表明,板栗花药发育过程中的营养物质的积累和运转有一定的时空特性:雄蕊原基时期,萼片组织中含有大量多糖物质;小孢子母细胞时期,花药壁组织上开始分布多糖物质;减数分裂为胞质同时型,同时药壁组织中出现淀粉,绒毡层细胞中含有蛋白质;四分体时期,花药壁和药隔组织中淀粉和多糖含量增加,绒毡层细胞内蛋白质增加;小孢子早期,小孢子细胞内出现淀粉粒,绒毡层蛋白质进一步增加;二核花粉时期,绒毡层只剩残迹,细胞开始积累大量淀粉、多糖以及蛋白质;成熟花粉粒为2-细胞型,属于淀粉型花粉,其中含有大量淀粉粒和少量多糖、蛋白质作为营养物质.
Histochemical Observation of Anthers Development in Castanea mollissima
The male flowers of Castanea mollissima at different developmental stages were used as experimental materials to observe the accumulation and distribution of starch,polysaccharide and protein in the anther develop-ment of C.mollissima by histochemical method.The results showed the distribution of accumulation and tran-slocation of nutrients during the anther development of C.mollissima had displayed certain temporal and spatial characteristics.At stage of stamen primordium stage,a lot of polysaccharides were accumulated in the differen-tiation of sepal tissues.At stage of microspore mother cells,anther wall began to distribution of polysaccharides.The meiosis was cytoplasmic simultaneous type,at same time,starches were appeared in anther wall tissue,and proteins were contained in tapetum cells at stage of meiosis.At stage of tapetal,there were starches and poly-saccharides in anther wall and connective tissue increased,and proteins in tapetal cells was increased.At stage of early microspore,starches grains appeared in microspores,and tapetal cells accumulate proteins gradually in-creased.At stage of bicellular pollen,tapetal were only remnant and the cells began to accumulate large amounts of starches,polysaccharides and proteins.The mature pollen grains were 2-celled type,belonged to starch type pollen,and a large number of starches and a small amount of polysaccharides and proteins were accumulated in the pollen as nutrients.

Castanea mollissimaAnther developmentStarchPolysaccharideProtein

陈萍、李遵、王璐、王溯、袁德义、邹锋

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中南林业科技大学,经济林培育与保护教育部重点实验室,长沙,410004

江西省森林资源保护中心,南昌,330038

板栗(Castanea mollissima) 花药发育 淀粉 多糖 蛋白质

国家自然科学青年基金湖南省自然科学基金湖南省重点研发计划项目

315005542018JJ38702018NK2043

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(6)
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