Molecular Mechanism of Chitosan Regulating Growth and Development of Hevea brasiliensis Muell.Arg.
In order to prove its promoting effect on the growth and development of rubber tree,foliar spraying method was used to treat the leaves of'Reyan73397'and'Reyan917'tissue culture seedling,and the expression pat-terns of genes related to the growth and development of rubber tree were analyzed.Furthermore,correlation analy-sis and principal component analysis were performed.The results showed that chitosan could significantly up regu-lated the expression levels of energy synthesis gene HbA TP,photosynthetic gene HbRbsS and reactive oxygen quench enzymes HbAPX and HbCuZnSOD.Correlation and principal component analysis showed that all the 12 genes of'Reyan73397'were positively correlated,and only one principal component was found.Among the 12 genes of'Reyan917',except HbMnSOD and HbCOXI were not correlated,the expression of other genes was posi-tively correlated,and there were two principal components.The results of this study showed that chitosan could significantly promote the growth and development of rubber tree and specific to rubber variety.Chitosan could be widely used as a carrier of chemical regulatory agents in stress resistance and disease resistance of rubber tree.It provides scientific basis for the research of growth and resistance mechanism,and provides technical guidance for the research and development of new cultivation techniques in rubber tree.
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