Identification and Expression Analysis of bZIP Gene Family in Brassica juncea
Basic leucine zipper(bZIP)is a family of transcription factors widely existing in eukaryotes.It is involved in the growth and development of many plants and the response process to biotic and abiotic stresses,and has a strong regulatory role in the expression of many downstream genes.The aim of this study is to understand the gene structure of bZIP gene family in mustard,the relationship among members and the response pattern under abiotic stress.A total of 81 BjubZIPs genes were identified from Brassica Database(BRAD)by bioinformatics method.It was found that 34.57％of them lack introns,and 59.26％of them have isoelectric point less than 7,which indicates that more than half of bZIP proteins are acidic proteins.Subcellular localization predicts that all genes are located in the nucleus.The 81 BjubZIPs were further divided into 6 subgroups corresponding to Arabidopsis genes.The motif number and gene structure of the same subgroup were highly similar.Using the transcriptome data of Brassica juncea in public database,the expression of BjubZIP gene family under drought and high temperature stress was analyzed.It was found that BjubZIP02,BjubZIP21,BjubZIP28,BjubZIP38,BjubZIP50,BjubZIP62 and BjubZIP59 in subgroup A were up-regulated under high temperature and drought stress.It is speculated that these seven genes may have similar functions to those of a subgroup genes in Arabidopsis thaliana and participate in the correspon-ding physiological processes under abiotic stress.The results of this study can provide a reference for the gene cloning of bZIP family members in mustard and the function research in stress response.
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