Transcriptome Analysis of Ethephon-treated Oncidium Petals and Discovery of Flower Senescence-related Genes
In order to characterize flower senescence-related genes and clarify the molecular mechanism of ethylene-regulated petal senescence,RNA sequencing and transcriptome analysis were performed with the petals of ethephon-treated Oncidium gower ramsey cut flowers prior to full opening.A total of 234 619 unigenes were obtained by sequencing.Using NR,GO,COG/KOG,KEGG and Swissprot databases,we annotated 31 027(77.22％),11 126(27.69％),10 152(25.27％),5 523(13.75％)and 18 830(46.86％)genes.Of all,7 319 differentially expressed genes(DEGs)were detected.In GO function enrichment analysis,4 622 DEGs were annotated into three functional groups:biological process,cell component and molecular function.The KEGG function enrichment analysis showed that the DEGs are mainly involved in 15 metabolic pathways,among which the DEGs were enriched most in plant hormone signal transduction pathways.A number of key component genes of plant hormone signal transduction and transcription factor genes that may play a role in Oncidium petal senescence of ethylene regulation were identified.The transcriptome data of Oncidium petal senescence of ethylene regulation obtained in this study is helpful to understand the function of flower senescence-related genes and reveal the molecular mechanism of flower senescence.
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万方数据
