摘要
为挖掘花衰老相关基因,揭示乙烯调控花瓣衰老的分子机制,利用乙烯利处理文心兰南茜种(Oncidium gowerramsey)盛开前期的切花花瓣,并进行RNA测序和转录组分析.测序结果共得到234 619个unigene.利用NR、GO、COG/KOG、KEGG和Swissprot数据库分别进行基因功能注释,分别注释到31 027(77.22％)、11 126(27.69％)、10 152(25.27％)、5 523(13.75％)和 18 830(46.86％)条 unigenes.检测到差异表达基因有7 319条.GO功能富集分析共有4 622条差异表达基因注释到生物过程、细胞组分和分子功能3个功能群组;KEGG功能富集分析将差异表达基因主要参与15个代谢通路,其中植物激素信号转导富集的基因最多.鉴定出一批可能在乙烯调控文心兰花瓣衰老中发挥作用的植物激素信号转导关键成分基因和转录因子基因.本研究所获得的乙烯调控文心兰花瓣衰老转录组数据,有助于了解花衰老相关基因功能和阐明花衰老分子机制.
Abstract
In order to characterize flower senescence-related genes and clarify the molecular mechanism of ethylene-regulated petal senescence,RNA sequencing and transcriptome analysis were performed with the petals of ethephon-treated Oncidium gower ramsey cut flowers prior to full opening.A total of 234 619 unigenes were obtained by sequencing.Using NR,GO,COG/KOG,KEGG and Swissprot databases,we annotated 31 027(77.22％),11 126(27.69％),10 152(25.27％),5 523(13.75％)and 18 830(46.86％)genes.Of all,7 319 differentially expressed genes(DEGs)were detected.In GO function enrichment analysis,4 622 DEGs were annotated into three functional groups:biological process,cell component and molecular function.The KEGG function enrichment analysis showed that the DEGs are mainly involved in 15 metabolic pathways,among which the DEGs were enriched most in plant hormone signal transduction pathways.A number of key component genes of plant hormone signal transduction and transcription factor genes that may play a role in Oncidium petal senescence of ethylene regulation were identified.The transcriptome data of Oncidium petal senescence of ethylene regulation obtained in this study is helpful to understand the function of flower senescence-related genes and reveal the molecular mechanism of flower senescence.
基金项目
海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC147)
海南省重点研发计划项目(ZDYF2020053)
五指山市科技和工业信息化局项目(WZSKTPXM2021005)
国家自然科学基金项目(31560573)
NETL
NSTL
万方数据