Cloning and Sequence Analysis of Two Carbon Glycosyltransferase Genes from Dendrobium huoshanense
In this study,the leaves of Dendrobium huoshanense were used as research materials,and two full-length UDP-glucose named 2-hydroxyflavanone C-glucosyltransferase(UFCGT)genes were obtained by polymerase chain reaction,and the proteins encoded by these two genes were analyzed and functionally predicted.The results showed that the sequence lengths of UFCGT1 and UFCGT2 genes were 1 022 bp and 1 187 bp,and the lengths of open reading frame(ORF)were 966 and 933 bp respectively.The encoded proteins were acidic,hydrophobic,and non-transmembrane proteins,which were similar to the Orchidaceae plants Dendrobium catenatum and Phalaenop-sis equestris and clustered together,and neither signal peptide nor transmembrane domain exists.UFCGT1 has 27 potential phosphorylation sites and 1 N-glycosylation site,and is localized to mitochondria.UFCGT2 has 15 potential phosphorylation sites but no glycosylation sites,and is located outside the cell membrane.This study pro-vides a reference for elucidating the biosynthetic pathway of flavonoid carbon glycosides.
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