霍山石斛两个碳糖基转移酶基因的克隆及序列分析
Cloning and Sequence Analysis of Two Carbon Glycosyltransferase Genes from Dendrobium huoshanense
陈畅 1蒲天珍 1李永华 1杨晓利 1张秀桥 1余坤 1龚玲1
作者信息
- 1. 湖北中医药大学药学院,武汉,430065
- 折叠
摘要
本研究以霍山石斛(Dendrobium houshanense)叶片为研究材料,利用聚合酶链式反应(polymerase chain reaction,PCR)获得两个 UDP-葡萄糖:2-羟基黄烷酮 C-糖基转移酶(2-hydroxyflavanone C-glucosyltran-sferase,UFCGT)全长基因,并对这两个基因编码的蛋白质进行分析和功能预测.结果表明,UFCGT1和UFCGT2基因序列长度分别为1 022和1 187 bp,开放阅读框(open reading frame,ORF)长度分别为966和933 bp,编码蛋白均为酸性、疏水性、非跨膜蛋白,与兰科(Orchidaceae)植物铁皮石斛(Dendrobium catenatum)、桃红蝴蝶兰(Phalaenopsis equestris)聚为一支,均无信号肽和跨膜域的存在.UFCGT1有27个潜在磷酸化位点和1个N-糖基化位点,定位于线粒体.UFCGT2有15个潜在的磷酸化位点而无糖基化位点,定位于细胞膜外.本研究为阐明黄酮碳苷类化合物生物合成途径提供一定的参考.
Abstract
In this study,the leaves of Dendrobium huoshanense were used as research materials,and two full-length UDP-glucose named 2-hydroxyflavanone C-glucosyltransferase(UFCGT)genes were obtained by polymerase chain reaction,and the proteins encoded by these two genes were analyzed and functionally predicted.The results showed that the sequence lengths of UFCGT1 and UFCGT2 genes were 1 022 bp and 1 187 bp,and the lengths of open reading frame(ORF)were 966 and 933 bp respectively.The encoded proteins were acidic,hydrophobic,and non-transmembrane proteins,which were similar to the Orchidaceae plants Dendrobium catenatum and Phalaenop-sis equestris and clustered together,and neither signal peptide nor transmembrane domain exists.UFCGT1 has 27 potential phosphorylation sites and 1 N-glycosylation site,and is localized to mitochondria.UFCGT2 has 15 potential phosphorylation sites but no glycosylation sites,and is located outside the cell membrane.This study pro-vides a reference for elucidating the biosynthetic pathway of flavonoid carbon glycosides.
关键词
霍山石斛/基因克隆/序列分析Key words
Dendrobium huoshanense/Gene cloning/Sequence analysis引用本文复制引用
基金项目
湖北中医药大学青苗计划(2016ZZX019)
出版年
2024
NETL
NSTL
万方数据