毛果杨20S蛋白酶体基因家族成员预测及生物信息学分析
Prediction and Bioinformatics Analysis of 20S Proteasome Gene Family Members in Populus trichocarpa
张亚飞 1曹丽娜 1樊昊驹 1严海川 1刘关君1
作者信息
- 1. 东北林业大学林学院,哈尔滨,150040
- 折叠
摘要
20S蛋白酶体是一类苏氨酸内肽酶,除了自身降解部分无序蛋白外,还可以与调节因子结合参与泛素依赖途径和非泛素依赖途径进行蛋白质的特异性降解,这对植物的正常生长至关重要.然而,关于毛果杨20S蛋白酶体基因家族成员数量及相关生物信息学分析还未见报道,本研究以拟南芥(Arabidopsis thaliana)20S蛋白酶体基因家族为参考,在毛果杨(Populus trichocarpa)中共鉴定出33个假定的20S蛋白酶体基因家族成员.生物信息学分析结果表明,该家族基因所编码蛋白大多数为碱性蛋白及亲水性蛋白,主要存在于细胞质中;蛋白序列中存在多个保守的甘氨酸残基;系统进化树将酵母(Saccharomyces cerevisiae)、拟南芥、毛果杨的20S蛋白酶体家族基因分为4个亚类;组织特异性表达分析显示大多数20S蛋白酶体基因在根和茎中高表达.该研究鉴定了其他基因家族成员并进行生物信息学分析,为探索毛果杨中20S蛋白酶体参与的生理过程及相应作用机制提供理论依据,同时也可为其他林木20S蛋白酶体基因家族的研究提供参考.
Abstract
20S proteasome is a kind of threonine endopeptidase.In addition to degradating some disordered proteins by self,20S proteasome mainly combines with regulatory factors,participating in ubiquitin-dependent and ubiquitin-independent pathways for protein-specific degradation,then involved in many physiological processes of life,which are very important for normal growth of plants.However,the members of 20S proteasome gene family and its bioinformatics analysis in Populus trichocarpa have not been reported.Based on the 20S proteasome gene family of Arabidopsis thaliana,33 putative members of 20S proteasome gene family were identified.It was found that most of the proteins encoded by 20S proteasome gene family were basic proteins,most of which were hydrophilic and mainly existed in cytoplasm.Many conserved glycine residues were contained in the proteins.According to the phylogenetic tree,the 20S proteasome genes of Saccharomyces cerevisiae,A.thaliana and P.trichocarpa were divided into four subgroups,and tissue-specific expression analysis showed that most 20S proteasome genes were highly expressed in roots and stems.The study identified its gene family members and conducted bioinformatics analysis,providing a theoretical basis for exploring the physiological processes and corresponding mechanisms of 20S proteasome in P.trichocarpa,and can also provide reference for the research of 20S proteasome gene family in other trees.
关键词
毛果杨(Populus/trichocarpa)/20S蛋白酶体/生物信息学分析Key words
Populus trichocarpa/20S proteasome/Bioinformatics analysis引用本文复制引用
基金项目
国家级大学生创新训练项目(202010225196)
出版年
2024
NETL
NSTL
万方数据