Genetic Diversity Analysis and Outcrossing Rate Estimation of Oat Based on SSR Molecular Markers
By using SSR method,the genetic diversity of 9 oat materials and the genetic differences among the offsprings on the same spikelet were analyzed.Then outcrossig rate of each material was estimated.The amplified polymorphic loci were 81,and the average polymorphic information content(PIC)was 0.485 2.Among the ma-terials,the mother plant from the same material,the offsprings from the same spikelet,the average coefficients were respectively 0.688 0,0.879 6 and 0.964 0.All materials were divided into 9 groups when the coefficient was 0.80.In this study,the outcrossing rate of oat was measured firstly in China.Based on SSR,the outcrossing rate of 9 oat materials tested range from 1.39％to 6.11％,the average is 3.79％.It was proved that oat is a self-pollinated plant with a certain proportion of outcrossing.The results will provide important references for maintaining the pu-rity of oats varieties and the research on cross breeding.
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