白鲜根腐病病原菌分离与鉴定
Isolation and Identification of Root Rot Pathogen on Dictamnus dasycarpus
沈宝宇 1孙文松 1张天静 1刘坤1
作者信息
- 1. 辽宁省经济作物研究所,辽阳,111000;辽宁省农业科学院药用植物研究所,辽阳,111000
- 折叠
摘要
为明确辽宁白鲜主要病害根腐病的发生危害及病原菌的种类,本研究于2019-2020年对辽宁省白鲜主产区本溪县、清原满族自治县等8个地区进行了根腐病危害情况定点跟踪调查.采用组织分离法对不同地区采集的病样进行分离、纯化,再依据柯赫氏法则进行致病性测定并结合形态学和分子生物学鉴定.结果表明,不同白鲜种植地区病害发生率及严重程度存在一定差异,其中病害发生最高的是本溪县和清原满族自治县,病害发生率分别达7.1％～9.2％和6.1％～7.2％.经鉴定,病原菌代表菌株BX69-1与NCBI中茄病镰刀菌(Fusariuwm solani)的多条rDNA-ITS序列同源性达100％,因此鉴定茄病镰刀菌是引起辽宁地区白鲜根腐病的病原真菌.本研究明确了辽宁省白鲜根腐病病原菌,为白鲜生产上病害防治和抗病育种提供理论依据.
Abstract
In order to identify the occurrence and damage of root rot and the types of pathogens of dictamnus(Dictamnus dasycarpus)in Liaoning Province,the study conducted a fixed-point follow-up survey of root rot dam-age in Benxi City and Qingyuan Manchu Autonomous in Liaoning Province from 2019 to 2020.The disease sam-ples collected from different areas were isolated and purified by tissue isolation method,and then the pathogenicity was determined according to Koch's law,combined with morphological and molecular biological identification.The results showed that there were some differences in the disease incidence and severity among different planting areas.The highest disease incidence was in Benxi City and Qingyuan Manchu Autonomous,with the disease inci-dence of 7.1％～9.2％and 6.1％～7.2％,respectively.The homology of the rDNA-ITS sequences between the repre-sentative strain BX69-1 and F.solani in NCBI was 100％.Therefore,F.solani was identified as the pathogen of root rot in Liaoning Province.This study provided a theoretical basis for disease control and disease resistance breeding in dictamnus production.
关键词
白鲜/根腐病/茄病镰刀菌/病原鉴定/BX69-1Key words
Dictamnus dasycarpus Turcz./Root rot/Fusarium solani/Pathogen indentification/BX69-1引用本文复制引用
基金项目
现代农业产业技术体系项目()
辽宁省农业科技自主创新资金项目(2020CGPY0403)
辽宁省农业科学院学科建设计划(2019DD144221)
出版年
2024
NETL
NSTL
万方数据