Isolation and Identification of Root Rot Pathogen on Dictamnus dasycarpus
In order to identify the occurrence and damage of root rot and the types of pathogens of dictamnus(Dictamnus dasycarpus)in Liaoning Province,the study conducted a fixed-point follow-up survey of root rot dam-age in Benxi City and Qingyuan Manchu Autonomous in Liaoning Province from 2019 to 2020.The disease sam-ples collected from different areas were isolated and purified by tissue isolation method,and then the pathogenicity was determined according to Koch's law,combined with morphological and molecular biological identification.The results showed that there were some differences in the disease incidence and severity among different planting areas.The highest disease incidence was in Benxi City and Qingyuan Manchu Autonomous,with the disease inci-dence of 7.1％～9.2％and 6.1％～7.2％,respectively.The homology of the rDNA-ITS sequences between the repre-sentative strain BX69-1 and F.solani in NCBI was 100％.Therefore,F.solani was identified as the pathogen of root rot in Liaoning Province.This study provided a theoretical basis for disease control and disease resistance breeding in dictamnus production.
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