Effects of H2O2 Seed Soaking on Root Growth and Antioxidant Enzyme Ac-tivities of Tartary Buckwheat(F.tataricum)Seedlings
Hydrogen peroxide(H2O2)is an exogenous signal substance involved in growth regulation,which helps maintain plant cell homeostasis.H2O2 seed soaking can promote the growth of seedling roots and has a concentra-tion effect in physiological and biochemical processes.In order to explore the effect of H2O2 seed soaking on Tar-tary buckwheat seedling root growth and to screen the appropriate concentration of H2O2 soaking to treat Tartary buckwheat seeds,this experiment used the cultivar of'Chuanqiao-2'as the material to study the effects of seed soaking with different H2O2 concentrations(1,5,10,20,50 and 100 mmol/L)on the root growth and antioxidant enzyme activty of Tartary buckwheat seedlings.The results showed that the internal root traits,root activity,CAT,POD and SOD activities of H2O2 at a concentration of 1～10 mmol/L were significantly higher than those of CK(P＜0.05).Except for RAD,other root traits and three antioxidant enzyme activities gradually decreases when the con-centration of H2O2 exceeded 20 mmol/L,and reaches a significant level.Through cluster analysis,most of the indi-cators within the concentration range of 1～10 mmol/L H2O2 reach their peaks,and each indicator is divided into two categories,one is RAD,RA,CAT and POD,and the remaining indicators are classified into another category.Correlation analysis shows that all indicators except RAD,RA,CAT and POD are extremely significantly positive-ly correlated.Principal component analysis shows that RL,RSA,RV,RF,RC,RFW and SOD can be used as the primary indicators for evaluating the root characteristics of Tartary buckwheat seedlings by H2O2 seed soaking,and RAD and POD can be used as secondary indicators.In summary,Soaking seeds with a concentration of 1～5 mmol/L H2O2 is beneficial to the growth of the seedling roots and root activity,and increase antioxidant enzyme activity of'Chuanqiao-2'.Based on the study results,the appropriate concentration and evaluation index of H2O2 soaking Tar-tary buckwheat seeds were screened out,and it provided a theoretical basis for the H2O2 soaking pretreatment to improve Tartary buckwheat seeds.
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