拉蔓期党参响应干旱胁迫生理机制
Physiological Mechanism of Codonopsis pilosula at Vine Growth Stage in Response to Drought Stress
栗锦鹏 1孙晓琛 1原静静 1杜弢 2王惠珍2
作者信息
- 1. 甘肃中医药大学药学院,兰州,730000
- 2. 甘肃中医药大学药学院,兰州,730000;甘肃中医药大学,西北中藏药协同创新中心,兰州,730000
- 折叠
摘要
为了探究拉蔓期党参响应干旱胁迫的生理机制,本研究采用室外盆栽控水试验,测定拉蔓期党参在不同干旱程度下土壤和叶片相对含水量、叶绿素、膜脂过氧化产物、渗透调节物质及抗氧化酶等生理生化指标变化.结果表明,随干旱加剧,拉蔓期党参叶片相对含水量逐渐降低;叶绿素含量逐渐增加;叶片相对电导率、超氧阴离子和丙二醛等膜脂过氧化指标均逐渐增加;渗透调节物质中脯氨酸和可溶性糖含量呈先增后降趋势,可溶性蛋白呈增加趋势;抗氧化酶(SOD,POD,CAT,APX)活性均呈先增后降趋势,其中CAT对干旱胁迫敏感程度更高.由此说明,拉蔓期党参主要通过可溶性蛋白来调节干旱胁迫下细胞渗透势,通过CAT降低干旱胁迫下膜脂过氧化对细胞的伤害.
Abstract
In order to explore the physiological mechanism of Codonopsis pilosula responding to drought stress,this study used an outdoor potted water control experiment to determine the relative water content of soil and leaves,chlorophyll,products of membrane lipid peroxidation,and osmotic adjustment substances under different drought levels in Codonopsis pilosula and changes in physiological and biochemical indicators such as antioxidant enzymes.The results showed that as the drought intensified,the relative water content of the leaves of Codonopsis pilosula gradually decreased;the chlorophyll content gradually increased;the relative electrical conductivity of the leaves,superoxide anion and malondialdehyde and other membrane lipid peroxidation indexes gradually in-creased;the content of amino acids and soluble sugars increased first and then decreased,while soluble proteins in-creased;the activities of antioxidant enzymes(SOD,POD,CAT and APX)all increased first and then decreased,and CAT is more sensitive to drought stress.This indicated that vine growth stage Codonopsis pilosula mainly reg-ulated cell osmotic potential under drought stress by soluble protein,and reduced the damage of membrane lipid peroxidation to cells under drought stress by CAT.
关键词
党参/叶绿素/膜脂过氧化/渗透调节物质/抗氧化酶Key words
Codonopsis pilosula/Chlorophylls/Membrane lipid peroxidation/Osmotic adjustment substance/An-tioxidant enzymes引用本文复制引用
基金项目
国家自然科学基金项目(81760683)
现代农业产业技术体系项目(CARS-21)
出版年
2024
NETL
NSTL
万方数据