Histocytological Observation and Physiological-Biochemical Differences of Somatic Embryogenesis in Akebia trifoliate
In this study,the non-embryog enic callus,embryogenic callus,early somatic embryos,and mature somatic embryos of A.trifoliate were used as materials to conduct histocytological observation and physiological and biochemical difference analysis of the whole embryogenesis of A.trifoliate.The results showed that embryonic and non-embryonic cells differed significantly in cell morphology,arrangement and starch granule distribution.Somatic embryogenesis of A.trilobata underwent four stages:globular embryogenesis,heart-shaped embryogenesis,torpedo embryogenesis and cotyledon embryogenesis,and it was observed that somatic embryogenesis was exogenous.The content of soluble protein,soluble sugar and antioxidant enzymes SOD,POD and CAT in embryogenic callus were higher than those of non-embryogenic callus.The cell activity and metabolism of em-bryogenic callus were higher than that of non-embryogenic callus.During the process of somatic embryogenesis,the content of soluble protein,soluble sugar and CAT activity showed an upward trend,and the accumulation of material energy was greater than the consumption,which provided the basis for the germination of regenerated seedlings,while the activities of antioxidant enzymes SOD and POD showed a downward trend,and its high activity was beneficial to somatic embryogenesis of A.trifolia.
A.trifoliaSomatic embryogenesisCytological observationPhysiology and biochemistry
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