干旱和镉复合胁迫对紫花苜蓿种子萌发的影响
Interactive Effects of Drought and Cadmium Stresses on Seed Germination of Alfalfa(Medicago sativa L.)
张书东 1尚敏 1凌立贞1
作者信息
- 1. 六盘水师范学院生物科学与技术学院,六盘水,553004
- 折叠
摘要
为了探究干旱和镉胁迫对紫花苜蓿种子萌发的影响,本实验以'中苜强'紫花苜蓿种子为实验材料,研究在不同胁迫浓度镉(0,1,5,10,50,100,200 mg/L)和 PEG(0％,5％,10％,15％,20％,25％,30％)以及复合胁迫(Cd,2.5～25.0 mg/L;PEG,2.5％～12.5％)下种子萌发的情况.结果表明:(1)在单一镉胁迫下,当浓度≥100 mg/L时对种子的萌发有明显的抑制作用;(2)在单一 PEG诱导的干旱胁迫下,浓度高于10％时对种子萌发有明显的抑制作用;(3)在镉/干旱复合胁迫下,Cd2+和PEG尽管未达到单因素胁迫浓度条件却对紫花苜蓿种子的萌发产生了明显的抑制作用,且随着它们各自浓度的增加,抑制作用逐渐增强.本实验结果可为六盘水和其他遭受重金属镉和干旱共同发生地区的植物种子萌发提供理论基础和借鉴意义.
Abstract
To explore the effect of polyethylene glycol(PEG)-induced drought and cadmium(Cd)stresses on seed germination of alfalfa(Medicago sativa L.),in this experiment,alfalfa seed of'medium-strong'was used as the expe-rimental material.First measured the seed germination,germination potential,germination index and vigor index at the different concentrations of Cd2+(0,1,5,10,50,100,200 mg/L)and PEG(0％,5％,10％,15％,20％,25％,30％).And then,the interactive effects of Cd2+(2.5～25.0 mg/L)and PEG(2.5％～12.5％)on seed germination also analy-zed.The results showed that:(1)The seed germination of alfalfa was apparently inhibited under 100 mg/L and hig-her concentration of Cd2+;(2)When the concentration of PEG was higher than 10％,the values of four germination indexes apparently decreased;(3)Interactive effects of PEG and Cd stress were very obvious even if the seeds of al-falfa were not exposed to their stress concentrations.The higher of the respective concentration of PEG and Cd2+,the stronger of inhibitory effects on seed germination.These results will provide a theoretical basis and reference for plant seed germination in Liupanshui and other areas suffering from heavy metal cadmium and drought stresses.
关键词
干旱胁迫/镉胁迫/复合胁迫/种子萌发Key words
Drought stress/Cadmium stress/Collaborative stress/Seed germination引用本文复制引用
基金项目
六盘水市科技计划项目(52020-2019-05-05)
六盘水市科技计划项目(52020-2022-PT-20)
大学生创新训练项目(S202010977004)
出版年
2024
NETL
NSTL
万方数据