Interactive Effects of Drought and Cadmium Stresses on Seed Germination of Alfalfa(Medicago sativa L.)
To explore the effect of polyethylene glycol(PEG)-induced drought and cadmium(Cd)stresses on seed germination of alfalfa(Medicago sativa L.),in this experiment,alfalfa seed of'medium-strong'was used as the expe-rimental material.First measured the seed germination,germination potential,germination index and vigor index at the different concentrations of Cd2+(0,1,5,10,50,100,200 mg/L)and PEG(0%,5%,10%,15%,20%,25%,30%).And then,the interactive effects of Cd2+(2.5~25.0 mg/L)and PEG(2.5%~12.5%)on seed germination also analy-zed.The results showed that:(1)The seed germination of alfalfa was apparently inhibited under 100 mg/L and hig-her concentration of Cd2+;(2)When the concentration of PEG was higher than 10%,the values of four germination indexes apparently decreased;(3)Interactive effects of PEG and Cd stress were very obvious even if the seeds of al-falfa were not exposed to their stress concentrations.The higher of the respective concentration of PEG and Cd2+,the stronger of inhibitory effects on seed germination.These results will provide a theoretical basis and reference for plant seed germination in Liupanshui and other areas suffering from heavy metal cadmium and drought stresses.