Bioinformatics and Expression Analysis of HaKASI Gene in Sunflower
KA SI encodes beta-ketoacyl-acyl carrier protein(ACP)synthase Ⅰ,which mainly plays a catalytic role in the reaction of C4-ACP to C16-ACP.In order to understand the expression characteristics and function of HaKASI gene in sunflower,the bioinformatics of the protein encoded by HaKASI and gene expression pattern were analyzed.It was predicted that HaKASI was acidic and hydrophilic.The secondary structure of the protein encoded by HaKASI was mainly random coil,and the subcellular localization was predicted in the chloroplast.Phylogenetic analysis showed that HaKASI clustered with KASI of Arabidopsis thaliana and other species in a large branch,and was closely related to Lactuca sativa L.KASI,and the sequence similarity was as high as 92％.The results of expres-sion analysis of HaKASI showed that the expression level of HaKASI was highest in sunflower seeds,followed by tubular flowers and stems,but not in leaves,ligulate flowers and roots.The expression trend of HaKASI in high and low oleic acid seeds at different stages of development was basically the same,and the expression level increased from 7 to 12 DAF and then decreased.But at 37 DAF,the expression level of HaKASI in low oleic acid seeds was extremely high,which was significantly higher than that in high oleic acid seeds.It suggests that HaKASI may have different expression regulation mechanisms at the late development stage of sunflower seeds with different oleic acid content.This study provides a scientific basis for the study of the function of HaKASI gene.
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