Characterization Analysis of Microsatellite in Chloroplast of Syringa reticu-lata subsp.amurensis
Syringa reticulata subsp.amurensis can be used as a decorative or a medicinal plant.In order to study the characteristics of chloroplast microsatellite,we analyzed the chloroplast genome sequence(156141 bp)by using MISA v1.0 software,and identified 231 microsatellite loci(one SSR locus occurred per 676 bp on average).Most of the genes distributed in the large single-copy region(94.52％).The cpSSR were mainly made out of A and T bases.The most common repeat type of the cpSSR was mononucleotide repeat,they accounted for 66.97％.The length of microsatellites maily distributed from 8～19 bp and accounted for 96.79％.There were 7 cpSSR of length equal to or greater than 20 bp,indicating that the cpSSR of S.reticulate had the potency of high polymorphism.Study on the cpSSR of S.reticulate could provide genetic markers for species identification and phylogentic studies.
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