摘要
暴马丁香具有很高的观赏价值和药用价值.为研究暴马丁香叶绿体微卫星特征,本研究在暴马丁香叶绿体全基因组序列(156 141 bp)的基础上,采用MISA v1.0软件,分析其叶绿体全基因组序列,共识别出231个微卫星位点(平均每676bp出现一个).其中,叶绿体微卫星主要集中于大单拷贝区(94.52％),主要由A、T构成.重复序列中主要以单碱基重复序列为主,占重复序列总数的66.97％.微卫星长度集中在8～19bp,占96.79％,有7个叶绿体微卫星长度大于20 bp,说明暴马丁香叶绿体基因组上的微卫星位点大多数具有多态性的潜能.实验结果为暴马丁香的物种鉴定和系统发育研究提供理论基础.
Abstract
Syringa reticulata subsp.amurensis can be used as a decorative or a medicinal plant.In order to study the characteristics of chloroplast microsatellite,we analyzed the chloroplast genome sequence(156141 bp)by using MISA v1.0 software,and identified 231 microsatellite loci(one SSR locus occurred per 676 bp on average).Most of the genes distributed in the large single-copy region(94.52％).The cpSSR were mainly made out of A and T bases.The most common repeat type of the cpSSR was mononucleotide repeat,they accounted for 66.97％.The length of microsatellites maily distributed from 8～19 bp and accounted for 96.79％.There were 7 cpSSR of length equal to or greater than 20 bp,indicating that the cpSSR of S.reticulate had the potency of high polymorphism.Study on the cpSSR of S.reticulate could provide genetic markers for species identification and phylogentic studies.
基金项目
青海省自然科学基金项目(2019-ZJ-984Q)
NETL
NSTL
万方数据