Transcriptome Analysis of Petal Color Formation of Meconopsis wilsonii
In order to understand the mechanism of flower color change in Meconopsis wilsonii,transcriptome se-quencing was performed on petals at bud stage,dehiscence stage and full expansion stage.Genes related to flower color change were screened out according to the characteristics of gene differential expression in each developmental stage.The differentially expressed genes were verified by fluorescence quantitative RT-qPCR.The results showed that 153 596 unigenes were generated by petal transcriptome sequencing,with an average length of 1 372 bp.Through the annotation of KEGG and GO database,134 genes were annotated into the synthetic pathway related to flower and color--the flavonoid metabolism pathway.A total of 80 240 CDS were generated by the related sequence prediction for unigene.Compared with bud stage,dehiscence stage and full spreading stage,15 064 genes were up-regulated and 49 153 genes were down-regulated in dehiscence stage,19 732 genes were up-regulated and 42 890 genes were down-regulated in full spreading stage,19 215 genes were up-regulated and 9 510 genes were down-regulated in full spreading stage compared with full spreading stage.By analyzing the metabolic pathways of the differentially expressed genes,it was found that delphinin synthesis pathway did not exist in the formation of the blue purple color of Meconopsis wilsonii.In addition,seven genes related to flower color in the anthocyanin syn-thesis pathway were screened,and RT-qPCR confirmed that the expression pattern of these genes during the whole process was consistent with the RNA-seq data.The purpose of this study is to deepen the cognition of the pattern formation of Meconopsis wilsonii and to provide theoretical basis for the future research on the pattern and color of Meconopsis vermosi.
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