Cloning and Expression Analysis of PmMADS13 Gene in Pinus massoniana
SOC1/TM3 is a member of the MADS-box family,which can mediate the signal integration of multi-ple flowering pathways to regulate flowering time and the development of floral organs.In order to understand the role of SOC1 genes in the reproductive growth of Pinus massoniana,a SOC1 homologous gene was screened from transcriptome sequencing results,and specific primers were designed.The rapid amplification of cDNA ends(RACE)technique was adopted to obtain the full-length cDNA sequence of the gene,which was 1 147 bp,includ-ing 666 bp open reading frame(ORF).A total of 220 amino acids were encoded and the gene was named as Pm-MADS13.The amino acid sequence alignment analysis showed that the protein contained typical MADS-box and K-box domains,with a highly conserved SOC1 motif at its C-terminal;phylogenetic tree analysis indicated that PmMADS13 gene of Pinus massoniana was most closely related to DAL9 gene of Pinus tabulaeformis,MADS9 gene of Pinus radiata and MADS13 gene of Pinus taeda,belonging to SOC1/TM3 subfamily;RT-PCR analysis showed that PmMADS13 was highly expressed in both male and female flowers of reproductive organs in Pinus massoni-ana,followed by expressing in stem tip and needle leaves,and had the lowest expression level in root tissues.It was speculated that PmMADS13 was an important gene involved in the flower formation of Pinus massoniana.
NETL
NSTL
万方数据
