不同居群蒙古扁桃的遗传多样性SRAP分析
SRAP Analysis of Genetic Diversity in Different Populations of Amygdalus mongolica
丁海麦 1白羽 1周红兵 1石松利1
作者信息
- 1. 内蒙古科技大学包头医学院,包头,014040
- 折叠
摘要
本研究选取5个具有代表性野生蒙古扁桃种群为研究对象,以同科近属蒙古柄扁桃种群为对照,采用SRAP分子标记方法,对珍稀濒危药用植物蒙古扁桃遗传多样性进行研究,为蒙古扁桃资源的合理保护和可持续利用提供科学的依据.用筛选出的12对SRAP引物,通过优化的反应体系对60个样品进行扩增,共扩增出171个清晰可重复的条带,其中多态性条带有166条,占97.08％.在物种水平上,Nei's基因多样度(h)0.371 1、Shannon信息指数(I)为0.546 8;种群间遗传分化系数(Gst)为0.596 4.结果显示蒙古扁桃居群的遗传变异主要存在于居群间,居群间的遗传分化程度大于居群内的,综合评价贺兰山的蒙古扁桃遗传多样性较高,应当优先保护.
Abstract
In this study,five different populations of A mygdalus mongolica were taken as the material research ob-ject,and the population of Prunus mongolica with the same genus and related species as the control.The genetic di-versity of rare and endangered medicinal plant Amygdalus mongolica was studied by SRAP molecular marker method,so as to provide a scientific basis for the rational protection and sustainable utilization of Amygdalus mon-golica resources.Using 12 pairs of SRAP primers screened,60 samples were amplified through the optimized reac-tion system.A total of 171 clear and repeatable bands were amplified,of which 166 were polymorphic,accounting for 97.08％.At the species level,Nei's gene diversity(h)was 0.371 1 and Shannon information index(I)was 0.546 8;The coefficient of genetic differentiation(Gst)among populations was 0.596 4.The results showed that the genet-ic variation of Amygdalus mongolica population mainly existed among populations,and the degree of genetic dif-ferentiation among populations was greater than that within populations.Through comprehensive evaluation,norikong had high genetic diversity and should be protected first.
关键词
蒙古扁桃/柄扁桃/SRAP/遗传多样性Key words
Amygdalus mongolica/Prunus mongolica/SRAP/Genetic diversity引用本文复制引用
基金项目
国家自然科学基金项目(81641137)
国家自然科学基金项目(81760782)
内蒙古自治区第十一批"草原英才"人才工程(2015MS0808)
内蒙古自治区第十一批"草原英才"人才工程(2019MS08189)
内蒙古自治区高等学校科学研究项目(NJZY21048)
出版年
2024
NETL
NSTL
万方数据