Development and Versatility Analysis of EST-SSR Markers Based on Tran-scriptome Sequence of Acer davidii
A cer davidii is one of the most important tree species for autumn color leaf landscaping.In this study,SSR marker analysis was performed based on the transcriptome data of Acer davidii leaves.Among them,3 744 unigene sequences contained 3 763 SSR sites,with a frequency of 6.93％,and each 11.89 kb contained one site.The dominant repeat sequence types are dinucleotide repeats and trinucleotide repeats,accounting for 51.21％and 46.93％of the total SSR sites respectively;AG/CT and GAA/TTC are the dominant dinucleotide and trinucleotide repeats,respectively.Among 654 primer pairs,252 pairs(38.53％)of primers can successfully amplify the target band,and 75 pairs(11.47％)of primers showed polymorphism.The transfer rate of 75 pairs of polymorphic primers among 16 germplasms of Acer genus ranged from 83.75％to 100％,and the average transfer rate was 90.08％,indicating that these 75 pairs of markers have high versatility.Using the transcriptome to develop SSR markers of A.davidii can provide important genetic resources for A.davidii and Acer germplasm resources re-search and molecular marker-assisted breeding.
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