脆木耳转录组EST-SSR标记特征及多态性分析
Characteristics and Polymorphism Analysis of EST-SSR Markers from Auri-cularia fibrillifera Transcriptome
王艺琴 1张素勤 1郭豪 1田怀志 1熊兴伟 1刘进平 1耿广东1
作者信息
摘要
为开发脆木耳EST-SSR标记,本试验从脆木耳转录组测序数据挖掘SSR位点,并设计引物对木耳DNA进行多态性分析,为木耳的遗传育种提供理论基础.结果发现,在脆木耳转录组的52 954条unigene序列中共搜寻到4 600个SSR位点,SSR发生频率为6.85％,分布频率为8.69％.脆木耳SSR位点共包括112种重复基元;其中,二核苷酸和三核苷酸重复为主要类型,占SSR总数的92.86％.三核苷酸重复最多(66.64％),其优势重复单元为CCG/GGC(8.71％).从设计引物中随机挑选30对进行PCR扩增,6对引物在3份木耳种质中均能扩增出清晰的目标条带.本试验所获得的EST-SSR可为木耳种质资源鉴定、遗传结构分析及遗传图谱构建等提供参考.
Abstract
In order to develop EST-SSR markers of Auriculus auriculatus,SSR loci were mined from transcrip-tome sequencing data of A.auriculatus,and primers were designed for polymorphism analysis of A.auriculatus DNA,providing theoretical basis for genetic breeding of A.auriculatus.The results showed that a total of 4 600 SSR loci were found in 52 954 unigenes of A.fibrillifera transcriptome,with 6.85％occurrence frequency and 8.69％distribution frequency,respectively.The SSR loci included 112 repeat motifs;among which dinucleotide and trinucleotide repeats were the main types,accounting for 92.86％among the total SSRs.The trinucleotide re-peats were most(66.64％),and dominant repeat unit was CCG/GGC(8.71％).30 pairs of primers were randomly selected for PCR amplification,and 6 pairs of them could amplify clear target bands in 3 Auricularia germplasms.The EST-SSR obtained in this experiment would provide reference for genetic structure analysis,germplasm re-source identification and genetic map construction in Auricularia.
关键词
脆木耳/EST-SSR/特征/PCR体系优化/多态性Key words
Auricularia fibrillifera/EST-SSR/Characteristics/Optimization of PCR system/Polymorphism引用本文复制引用
基金项目
贵州省科技计划项目(黔科合重大专项字[2019]3005-5)
贵州省科技计划项目(黔科合成果[2020]1Z002号)
贵州省科技计划项目(黔科合成果[2021]一般063)
凤冈县辣椒博士工作站建设项目()
国家重点研发计划项目(2021YFD1100301)
出版年
2024
NETL
NSTL
万方数据