首页|脆木耳转录组EST-SSR标记特征及多态性分析

脆木耳转录组EST-SSR标记特征及多态性分析

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为开发脆木耳EST-SSR标记,本试验从脆木耳转录组测序数据挖掘SSR位点,并设计引物对木耳DNA进行多态性分析,为木耳的遗传育种提供理论基础.结果发现,在脆木耳转录组的52 954条unigene序列中共搜寻到4 600个SSR位点,SSR发生频率为6.85％,分布频率为8.69％.脆木耳SSR位点共包括112种重复基元;其中,二核苷酸和三核苷酸重复为主要类型,占SSR总数的92.86％.三核苷酸重复最多(66.64％),其优势重复单元为CCG/GGC(8.71％).从设计引物中随机挑选30对进行PCR扩增,6对引物在3份木耳种质中均能扩增出清晰的目标条带.本试验所获得的EST-SSR可为木耳种质资源鉴定、遗传结构分析及遗传图谱构建等提供参考.
Characteristics and Polymorphism Analysis of EST-SSR Markers from Auri-cularia fibrillifera Transcriptome
In order to develop EST-SSR markers of Auriculus auriculatus,SSR loci were mined from transcrip-tome sequencing data of A.auriculatus,and primers were designed for polymorphism analysis of A.auriculatus DNA,providing theoretical basis for genetic breeding of A.auriculatus.The results showed that a total of 4 600 SSR loci were found in 52 954 unigenes of A.fibrillifera transcriptome,with 6.85％occurrence frequency and 8.69％distribution frequency,respectively.The SSR loci included 112 repeat motifs;among which dinucleotide and trinucleotide repeats were the main types,accounting for 92.86％among the total SSRs.The trinucleotide re-peats were most(66.64％),and dominant repeat unit was CCG/GGC(8.71％).30 pairs of primers were randomly selected for PCR amplification,and 6 pairs of them could amplify clear target bands in 3 Auricularia germplasms.The EST-SSR obtained in this experiment would provide reference for genetic structure analysis,germplasm re-source identification and genetic map construction in Auricularia.

Auricularia fibrilliferaEST-SSRCharacteristicsOptimization of PCR systemPolymorphism

王艺琴、张素勤、郭豪、田怀志、熊兴伟、刘进平、耿广东

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贵州大学农学院,贵阳,550025

脆木耳 EST-SSR 特征 PCR体系优化 多态性

贵州省科技计划项目贵州省科技计划项目贵州省科技计划项目凤冈县辣椒博士工作站建设项目国家重点研发计划项目

黔科合重大专项字[2019]3005-5黔科合成果[2020]1Z002号黔科合成果[2021]一般0632021YFD1100301

2024

10.13271/j.mpb.022.002611

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子：0.765
ISSN：1672-416X
年,卷(期)：2024.22(8)
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