Identification of Pathogenic of Torreya grandis'Merrillii'Root Rot Disease
Clarify the type of pathogen of T.grandis'Merrillii'root rot disease.The isolated pathogens were tested for pathogenicity and the classification status of the pathogens was determined by using molecular techniques com-bined with morphological identification.T.grandis'Merrillii'disease strains were isolated from tissues,and 12 strains were isolated and purified.After molecular biological identification,these 12 strains were classified into 10 categories,represented by ZJ-1 to ZJ-10.The pathogenicity test of ZJ-1 to ZJ-10 was carried out,and according to Kocher's law,it was confirmed that ZJ-10 is the pathogen of T.grandis'Merrillii'root rot disease.ZJ-10 strain was identified as Fusarium solani by combining morphological characteristics and ITS rDNA sequence.The pathogen causing T.grandis'Merrillii'root rot is Fusarium solani.
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