In order to improve the plant regeneration rate of rose somatic embryos genetically transformed,the rose material B1 03(Rosa spp.)sterile seedling was used as the material,explore the effects of different concentra-tions of cytokinin 6-BA,KT,and TDZ on plant growth,and use the optimized medium RB and RT to induce re-generation of mature rose material B103 somatic embryos.The results showed that the sterile seedlings on the medium(RK)supplemented with KT all died,and the two groups supplemented with 6-BA(RB)and TDZ(RT)grew normally.The best medium is MS+NAA 0.05 mg/L+GA3 3.0 mg/L+6-BA/TDZ 0.5 mg/L+3％sucrose+0.7％agar pH=5.7.Mature rose somatic embryos were induced to differentiate on the medium MS+NAA 0.05 mg/L+GA3 3.0 mg/L+TDZ 0.5 mg/L+3％sucrose+0.7％agar pH=5.7,the largest number of normal plants regenerated within 30 days,with a regeneration rate of 20％.Cotyledons in a clumped state were formed by RT components,and the regeneration rate was higher than 20％.This study showed that ET2 could induce the differentiation of rose cells into normal regenerated plants.
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