Effect of Three Plant Growth Regulators on in Vitro Regeneration of Rose spp.
In order to improve the plant regeneration rate of rose somatic embryos genetically transformed,the rose material B1 03(Rosa spp.)sterile seedling was used as the material,explore the effects of different concentra-tions of cytokinin 6-BA,KT,and TDZ on plant growth,and use the optimized medium RB and RT to induce re-generation of mature rose material B103 somatic embryos.The results showed that the sterile seedlings on the medium(RK)supplemented with KT all died,and the two groups supplemented with 6-BA(RB)and TDZ(RT)grew normally.The best medium is MS+NAA 0.05 mg/L+GA3 3.0 mg/L+6-BA/TDZ 0.5 mg/L+3％sucrose+0.7％agar pH=5.7.Mature rose somatic embryos were induced to differentiate on the medium MS+NAA 0.05 mg/L+GA3 3.0 mg/L+TDZ 0.5 mg/L+3％sucrose+0.7％agar pH=5.7,the largest number of normal plants regenerated within 30 days,with a regeneration rate of 20％.Cotyledons in a clumped state were formed by RT components,and the regeneration rate was higher than 20％.This study showed that ET2 could induce the differentiation of rose cells into normal regenerated plants.
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