Observation on Morphology of Flower Bud Differentiation and the Effect of Shading on the Process of Rosa
In order to study the process of flower bud differentiation and the effect of shading on the process of flower bud differentiation of rose,this experiment took'Rouge Meilland'as the experimental material,and used testing of stereomicroscopes with paraffin method to observe the morphology of flower bud differentiation process under normal light and shading(50％).The results showed that the flower bud differentiation lasted for 40 days,and the process could be divided into the beginning of differentiation,sepal primordium differentiation phase,petal primordium differentiation phase,stamen primordium differentiation phase,pistil primordium differentiation phase,pistil primordium differentiation phase.Among them,the repeatedly differentiation of petal primordium is the reason for the multiple corolla.Different flower organ originated from meristematic cell at the edge of the floral organelle primordium.The results showed that shading delayed the process of flower bud differentiation for about 7 days,and resulted in double flowers,reduced stamens and pistils,flower deformity and smaller flower diameter of some flowers.The two methods are consistent in the observation process,stereo microscope is more convenient and fast,it can better reflect the space utilization,while the paraffin method is complicated,but it can reflect the arrangement of cells with high definition.
Chinese roseFlower bud differentiationParaffin methodStereo microscopeShade
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