Heterologous Expression of Begonia semperflorens BsRbohD Gene Promotes Anthocyanin and Accumulation under High Light
In order to explore the function of the BsRbohD gene of Begonia semperflorens,the'Columbia'wild-type Arabidopsis thaliana heterologously expressing the BsRbohD gene of B.semperflorens was used as the material,an-alyzing the relationship between BsRbohD gene and anthocyanin through changing in maximum photochemical ef-ficiency(Fv/Fm),reactive oxygen species(ROS)levels,anthocyanin content and related synthetic gene expression.The results showed that the BsRbohD gene of B.semperflorens was expressed normally after being heterologously transferred into Arabidopsis thaliana.Under control conditions,two transgenic lines(OE1 and OE2)did not ex-hibit significant differences in Fv/Fm,anthocyanin content and anthocyanin-biosynthesis-related genes expression,although slight higher content of drogen peroxide(H2O2)and production rate superoxide anion(O2·-)were detected in OE1 and OE2.Under high light conditions,OE1 and OE2 showed higher expression level in BsRbohD,which consequently increased reactive oxygen species(ROS)level in OE1 and OE2 compared to WT;moreover,an-thocyanin-biosynthesis-related genes showed quicker and more upregulation in OE1 and OE2,which consequently accumulated more anthocyanins than WT under high light conditions.These findings suggested that ectopic over-expression of BsRbohD in A.thaliana produced more ROS which triggered anthocyanin biosynthesis through up-regulating anthocyanin-biosynthesis-related genes expression in OE1 and OE2.These findings provided theoretical support for further clarifying the function of BsRbohD gene.
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