Cloning of RuEG Genes from Blackberry and Its Expression Characteristics Under Different Postharvest Treatments
Blackberry fruit is soft and juicy with poor storability during postharvest period.Therefore,it is of practical significance to investigate the genes regulating this trait.Endoglucanase(endo-1,4-β-glucanase,EG)is an important enzyme in cellulase,which can decompose cell wall and affect fruit ripening and softening.Based on the results of transcriptome sequencing of blackberry'Hull'treated with ferulic acid and natamycin after harvest,two differentially expressed EG genes were cloned and named RuEG1 and RuEG2.In addition,the expression of these two genes in treated samples was analyzed.The results showed that RuEG1 and RuEG2 contained 1 578 bp and 1 491 bp open reading frame,which encoded 525 and 496 amino acids,respectively.RuEG1 and RuEG2 pro-teins belong to glycosylhydrolase family 9,containing four cysteine(Cys)residues,two amino acid conserved re-gions and one promoter site.The significant decrease in the expression of RuEG1 and RuEG2 genes in mature blackberry indicated that the expression of these genes was inhibited under postharvest treatment with ferulic acid and natamycin.This study will be a foundation for further study on the structure and function of EG genes as well as being of great significance to explore the role of EG enzyme in fruit ripening and softening.
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